Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgR Downstream Fragment in pUC18/7.24.08 Master Mix Second Round
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7.24.08 PCR Master Mix For Second Round of Screening (pKn004.L3 Only)
Reagent | Volume/Amount |
Colony Suspension | 0 μl |
Taq | 1.23 μl |
M13 for | 9.84 μl 1 μM Stock |
BT1188 | 9.84 μl 1 μM Stock |
dNTPs | 2.43 (oops, should have been 2.46) μl 10 mM Stock |
DMSO | 6.15 μl |
Buffer | 12.3 μl |
Mg2+ | 6.15 μl |
dH20 | 62.73 μl |
Total | 122.97 μl (missed 0.03 in dNTPs) |
Aliquot 9 μl to each of 12 tubes, then add 1 μl colony suspension to each.