Matt Gethers/20.380 HIV Project/Meeting Notes/3.4.08 (Final Cut on Design Ideas): Difference between revisions
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*Can HIV infect progenitor cells? - '''Matt''' | *Can HIV infect progenitor cells? - '''Matt''' | ||
*How to couple CD4 expression, etc with RBC differentiation? - '''Rob''' | *How to couple CD4 expression, etc with RBC differentiation? - '''Rob''' | ||
*Malaria - ''' | *Malaria - '''Courtney''' | ||
*What's necessary and sufficient for HIV infection? - '''Matt''' | *What's necessary and sufficient for HIV infection? - '''Matt''' | ||
*What to do with HIV once in cell? Do we need it to do anything? - '''Rob''' | *What to do with HIV once in cell? Do we need it to do anything? - '''Rob''' |
Revision as of 18:10, 10 March 2009
3.4.08 (Final Cut on Design Ideas)
To Do
Make list of issues/questions to consider in implementing the engineered T-Cell idea.
Make list of issues/questions to consider in implementing the engineered RBC idea.
Questions
- What do we need to put into the person? What stage of progenitor cells? - (done!)
- Immunity issues with progenitor cells? - Rob
- How are RBCs removed/recycled from body? - Courtney
- Can HIV infect progenitor cells? - Matt
- How to couple CD4 expression, etc with RBC differentiation? - Rob
- Malaria - Courtney
- What's necessary and sufficient for HIV infection? - Matt
- What to do with HIV once in cell? Do we need it to do anything? - Rob
- How to prevent HIV from leaving RBC? - Jessie
- Death switch? - Jessie
- Exploding RBCs? - David
- Any natural conditions where RBCs die or explode? - David
- Take out hemoglobin? Anything else to knock out? - Yi
- Pathologies of RBCs - esp. sickle cell anemia, thalassemia - Yi
- Ideal concentration of RBC progenitors? - Steph
- How to get into bone marrow? Easy injection method? - Steph
- Role of progenitors? - Steph
Make list of issues/questions to consider in implementing the superinfection idea.
Questions
- Does superinfection apply across strains?
- Fidelity of superinfection? How often does it fail?
- Matt
- Divide work for presentation next week (3/12/09)
- Modify calendar to reflect changes in schedule.
Minutes
Follow Up
Erythropoiesis
- Cell Lineage Map
- Need to start with hematapoeic stem cell
- knock out other lineages? (maybe we could leave them? would it be a problem?)
- Need to start with hematapoeic stem cell