Matt Gethers: Difference between revisions
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| IV||Separate from genes I and XI | | IV||Separate from genes I and XI | ||
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| M13 ORI 1|| | | M13 ORI 1 & 2|| Are two ORIs necessary? If so, can they be consolidated? | ||
|- | |- | ||
| KanR|| | | KanR|| | ||
|- | |-} | ||
Extractions, separations, etc. include ensuring that each gene has its own promoter, RBS, terminator, and any other pertinent regulatory sequences. | Extractions, separations, etc. include ensuring that each gene has its own promoter, RBS, terminator, and any other pertinent regulatory sequences. | ||
In addition to separating the genes, it would also be wise to develop or utilize/modify an existing system like [[ ||Biobricks]] to build the genome so that convenient restriction sites exist between the genes so they can be easily removed or replaced. | In addition to separating the genes, it would also be wise to develop or utilize/modify an existing system like [[ ||Biobricks]] to build the genome so that convenient restriction sites exist between the genes so they can be easily removed or replaced. | ||
Revision as of 22:31, 13 February 2007
I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.
20.109 Work
M13 Engineering Ideas
Extractions, separations, etc. include ensuring that each gene has its own promoter, RBS, terminator, and any other pertinent regulatory sequences. In addition to separating the genes, it would also be wise to develop or utilize/modify an existing system like [[ ||Biobricks]] to build the genome so that convenient restriction sites exist between the genes so they can be easily removed or replaced.| Gene | Modification |
| X | Extract from gene II |
| II | Extract gene X |
| V | |
| VII | Separate from gene IX |
| III | Change the GTG to ATG Start? |
| VI | |
| I | Separate from genes IV and XI |
| XI | Separate from genes I and IV |
| IV | Separate from genes I and XI |
| M13 ORI 1 & 2 | Are two ORIs necessary? If so, can they be consolidated? |
| KanR |
