Mathies:Valves - Revision history

Eric Chu at 05:07, 23 July 2009

2009-07-23T05:07:18Z

←Older revision		Revision as of 05:07, 23 July 2009
Line 93:		Line 93:

	[[Image:pumpaction1.jpg]]		[[Image:pumpaction1.jpg]]
		+	[[Image:pumpaction2.jpg]]
		+	[[Image:pumpaction3.jpg]]
		+
		+	To see the pumping sequence in video, click [http://www.cchem.berkeley.edu/ramgrp/protocol/pumpanimation.gif here].

	==References==		==References==

Eric Chu at 05:04, 23 July 2009

2009-07-23T05:04:53Z

←Older revision		Revision as of 05:04, 23 July 2009
Line 89:		Line 89:

	[[Image:valvesassembly_1.jpg]]		[[Image:valvesassembly_1.jpg]]
		+
		+	==Valve Operating Animations==
		+
		+	[[Image:pumpaction1.jpg]]

	==References==		==References==
Line 100:		Line 104:

	==Contact==		==Contact==
-	*'''[[User:Eric Chu\|Eric Chu]] 17:15, ~~21 May~~ 2009 (PDT)''':	+	*'''[[User:Eric Chu\|Eric Chu]] 22:10, 22 July 2009 (PDT)''':
	*'''[[User:Samantha Cronier\|Samantha Cronier]] 19:25, 9 April 2009 (EDT)''':		*'''[[User:Samantha Cronier\|Samantha Cronier]] 19:25, 9 April 2009 (EDT)''':

Eric Chu at 00:18, 22 May 2009

2009-05-22T00:18:04Z

←Older revision		Revision as of 00:18, 22 May 2009
Line 14:		Line 14:

	[http://www.rogerscorporation.com/hpf/IL/PDF/180-076%20HT-6240%20Liquid%20Rubber%20Sheeting.pdf PDMS information]		[http://www.rogerscorporation.com/hpf/IL/PDF/180-076%20HT-6240%20Liquid%20Rubber%20Sheeting.pdf PDMS information]
-	~~PDMS information~~

	==Sample Parts List==		==Sample Parts List==

Eric Chu at 00:16, 22 May 2009

2009-05-22T00:16:45Z

←Older revision		Revision as of 00:16, 22 May 2009
Line 5:		Line 5:


-	~~[[Image:valvesassembly_1.jpg]]~~	+	==Overview==

		+	This protocol includes all the parts to build the Mathies group Valves and Control System.

		+	==Materials==

		+	[http://www.stockwell.com/newstuff/newpdfs/500_solid_silicone/rogers_bisco_ht-6000_series_comparitive_chart.pdf PDMS Supply and properties]

-	~~==Overview==~~	+	[http://www.rogerscorporation.com/hpf/IL/PDF/180-076%20HT-6240%20Liquid%20Rubber%20Sheeting.pdf PDMS information]
		+	PDMS information

-	~~This protocol includes all the parts to build the Mathies group Valves and Control System.~~	+	==Sample Parts List==

-	~~==Materials==~~	+	3.5" x 2.7" miniboard of '''PCB Board''' from www.expresspcb.com
-	~~List reagents, supplies and equipment necessary to perform the protocol here~~. ~~For those materials which have their own OWW pages, link to that page~~. ~~Alternatively, links to the suppliers~~' ~~page on that material are also appropriate~~.	+

-	*supply 1 (i.~~e. tubes of a certain size? spreaders?)~~	+	You can download their design software, and submit through the software to get a design file.
-	*reagent 1	+
-	*X μL reagent 2	+
-	**component A (reagent 2 is made up of multiple components)	+
-	**component B	+
-	*equipment 1	+
-	*equipment 2	+

-	~~==Procedure==~~	+	Here's the image of PCB circuit board design:
-	~~#Step 1~~	+
-	~~#Step 2~~	+
-	~~#*Step 2 has some additional information that goes with it. i.e. Keep at 4°C.~~	+
-	~~#Step 3~~	+
-	~~##Step 3 has multiple sub-steps within it.~~	+
-	~~##Enumerate each~~ of ~~those.~~	+

-	~~==Notes==~~	+	[[Image:pcbimage1.jpg]]
-	~~Please feel free to post comments, questions, or improvements to this protocol~~. ~~Happy to have your input!~~	+
-	~~#List troubleshooting tips here.~~	+
-	~~#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.~~	+
-	~~#Anecdotal observations that might be of use to others can also be posted here.~~	+

-	~~Please sign your name~~ to ~~your note by adding <font face=~~"~~courier~~"~~><nowiki>~~'''*~~~~''':</~~nowiki><~~/~~font> to the beginning of your tip~~.	+	[[Image:pcbimage2.jpg]]
		+
		+	[[Image:pcbimage3.jpg]]
		+
		+
		+	'''Pump''' from Nor-Cal Controls (408)727-5756 www.norcal4air.com
		+
		+	12V Thomas 7011 Diaphragm Pump
		+
		+
		+	'''Solenoids''' from Teco Pneumatic (925)426-8500 www.tecopneumatic.com
		+
		+	8 port manifold for solenoids (need three for 24 lines)
		+
		+	5V DC Solenoid Valve for vacuum (as many lines as you need, 330 mA each)
		+
		+	M3 Barbed fitting to 1/8" tubing (as many lines as you need)
		+
		+
		+
		+	'''Electronics''' from Jameco (800)831-4242 www.jameco.com
		+
		+	5V@12A and 12V@5A switching AC to DC power supply
		+
		+	3 pin connector housing (for 5V power connection to board, bags of 30, only need a couple)
		+
		+	8 pin connector housing (for solenoid connections to board, bags of 30, only need a couple)
		+
		+	16 pin header straight (for TTL connection to PCB board)
		+
		+	16 pin connector (attaches to ribbon and fits in header)
		+
		+	Fuse resettable, 3.0 A-H 6.0 A-T (you may want to change the fuses depending on the number of solenoids and thpump used)
		+
		+	18 pin low profile IC socket
		+
		+	Darlington chip ULN2803A
		+
		+	2 pin connector housing (for 12V power connection to board)
		+
		+	0.100" Male Breakaway Headers straight single row
		+
		+	0.100" Male Breakaway Headers straight double row
		+
		+	0.000" Female crimp pin (fits in the connector housings after crimping on end of wire)
		+
		+	Ribbon cable (50 line)
		+
		+
		+
		+	'''Control Cards''' from National Instruments
		+
		+	NI-PCI-6503 24 line digital I/O
		+
		+
		+	==Sample Labview Program for Pumping==
		+
		+	Pneumatic pumps are controlled by Labview programs. Here's a sample pump program (zip file).
		+
		+	[http://www.cchem.berkeley.edu/ramgrp/protocol/Pump.zip Pump.zip]
		+
		+	==Assembling Valves Systems==
		+
		+	[[Image:valvesassembly_1.jpg]]

	==References==		==References==
-	~~'''Relevant papers and books'''~~	+
-	~~<!-- If this~~ protocol ~~has papers or books associated with it, list those references here~~. ~~See the [~~[~~OpenWetWare~~:~~Biblio]] page for more information~~. ~~-->~~	+	Papers related to Valves
-	~~<biblio>~~	+
-	~~#Goldbeter-PNAS-1981 pmid=6947258~~	+	[http://www.cchem.berkeley.edu/ramgrp/protocol/PDMSvalve.pdf 1 Original PDMS valve paper]
-	~~#Jacob-JMB-1961 pmid=13718526~~	+	[http://www.cchem.berkeley.edu/ramgrp/protocol/Latching.pdf 2 Latching valve paper]
-	~~#Ptashne-Genetic-Switch isbn=0879697164~~	+	[http://www.cchem.berkeley.edu/ramgrp/protocol/Computing.pdf 3 Computing paper]
-	</~~biblio>~~	+

	==Contact==		==Contact==
		+	*'''[[User:Eric Chu\|Eric Chu]] 17:15, 21 May 2009 (PDT)''':
	*'''[[User:Samantha Cronier\|Samantha Cronier]] 19:25, 9 April 2009 (EDT)''':		*'''[[User:Samantha Cronier\|Samantha Cronier]] 19:25, 9 April 2009 (EDT)''':

Eric Chu at 23:50, 21 May 2009

2009-05-21T23:50:38Z

←Older revision		Revision as of 23:50, 21 May 2009
Line 12:		Line 12:
	==Overview==		==Overview==

-	This protocol ~~explains how~~ to ~~fabricate microvalves on a glass chip with a PDMS manifold~~.	+	This protocol includes all the parts to build the Mathies group Valves and Control System.

	==Materials==		==Materials==

Samantha Cronier at 23:25, 9 April 2009

2009-04-09T23:25:25Z

←Older revision		Revision as of 23:25, 9 April 2009
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-
	[[Category:Protocol]]		[[Category:Protocol]]
	[[Category:Microfluidics]]		[[Category:Microfluidics]]

	<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! -->		<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! -->
		+
		+
		+	[[Image:valvesassembly_1.jpg]]
		+
		+
		+

	==Overview==		==Overview==

-	~~Replace this sentence~~ with a ~~brief description of the protocol and its goal~~.	+	This protocol explains how to fabricate microvalves on a glass chip with a PDMS manifold.

	==Materials==		==Materials==
Line 46:		Line 51:

	==Contact==		==Contact==
-	*~~Who has experience with this protocol?~~	+	*'''[[User:Samantha Cronier\|Samantha Cronier]] 19:25, 9 April 2009 (EDT)''':

	or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].		or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].
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	[[Category:Protocol]]		[[Category:Protocol]]
		+	[[Category:Microfluidics]]

Samantha Cronier at 22:54, 9 April 2009

2009-04-09T22:54:43Z

←Older revision		Revision as of 22:54, 9 April 2009
Line 1:		Line 1:

		+	[[Category:Protocol]]
		+	[[Category:Microfluidics]]

-
-
-
-	~~'''Interested in posting a protocol on OpenWetWare? Here is a template to help you do so.'''~~
-
-	'''Click the view source tab and copy everything below this line. Paste it into your new protocol page. Then replace the text in this page with your own protocol. Feel free to add or delete sections as appropriate.'''
-	~~[[Category:Template]]~~
	<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! -->		<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! -->

Samantha Cronier at 22:48, 9 April 2009

2009-04-09T22:48:14Z

←Older revision		Revision as of 22:48, 9 April 2009
Line 1:		Line 1:
-	~~<center>~~
-	~~{\|style="width: 48em; background: #FF6677;"~~
-	\| align="center"\|'''This page is a template and should not be edited.'''<br><span style="font-size:90%">Click [http://openwetware.org/index.php?title={{PAGENAME}}&action=edit here], copy the source, and paste it into your page.</span>
-	\|}
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-	~~<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Microfluidics]]-->~~
-	~~{{#dpl:category=Microfluidics\|category=Protocol}}~~
-	}}


Line 66:		Line 56:

	<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->		<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->
-	~~{{#dpl~~:~~category=Microfluidics\|category=~~Protocol}}	+
		+	[[Category:Protocol]]
		+
		+
	<!-- Move the relevant categories above this line to tag your protocol with the label		<!-- Move the relevant categories above this line to tag your protocol with the label
	[[Category:Protocol]]		[[Category:Protocol]]

Samantha Cronier at 22:45, 9 April 2009

2009-04-09T22:45:19Z

←Older revision		Revision as of 22:45, 9 April 2009
Line 4:		Line 4:
	\|}		\|}
	</center>		</center>
		+
		+	{{protocols group\|Microfluidics\|2=
		+	<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Microfluidics]]-->
		+	{{#dpl:category=Microfluidics\|category=Protocol}}
		+	}}


Line 61:		Line 66:

	<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->		<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->
-		+	{{#dpl:category=Microfluidics\|category=Protocol}}
	<!-- Move the relevant categories above this line to tag your protocol with the label		<!-- Move the relevant categories above this line to tag your protocol with the label
	[[Category:Protocol]]		[[Category:Protocol]]

Samantha Cronier at 22:40, 9 April 2009

2009-04-09T22:40:56Z

←Older revision		Revision as of 22:40, 9 April 2009
Line 1:		Line 1:
-	Here is the protocol for ~~Valves~~.	+	<center>
		+	{\|style="width: 48em; background: #FF6677;"
		+	\| align="center"\|'''This page is a template and should not be edited.'''<br><span style="font-size:90%">Click [http://openwetware.org/index.php?title={{PAGENAME}}&action=edit here], copy the source, and paste it into your page.</span>
		+	\|}
		+	</center>
		+
		+
		+
		+
		+
		+	'''Interested in posting a protocol on OpenWetWare? Here is a template to help you do so.'''
		+
		+	'''Click the view source tab and copy everything below this line. Paste it into your new protocol page. Then replace the text in this page with your own protocol. Feel free to add or delete sections as appropriate.'''
		+	[[Category:Template]]
		+	<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! -->
		+
		+	==Overview==
		+
		+	Replace this sentence with a brief description of the protocol and its goal.
		+
		+	==Materials==
		+	List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.
		+
		+	*supply 1 (i.e. tubes of a certain size? spreaders?)
		+	*reagent 1
		+	*X μL reagent 2
		+	**component A (reagent 2 is made up of multiple components)
		+	**component B
		+	*equipment 1
		+	*equipment 2
		+
		+	==Procedure==
		+	#Step 1
		+	#Step 2
		+	#*Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
		+	#Step 3
		+	##Step 3 has multiple sub-steps within it.
		+	##Enumerate each of those.
		+
		+	==Notes==
		+	Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
		+	#List troubleshooting tips here.
		+	#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
		+	#Anecdotal observations that might be of use to others can also be posted here.
		+
		+	Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
		+
		+	==References==
		+	'''Relevant papers and books'''
		+	<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. -->
		+	<biblio>
		+	#Goldbeter-PNAS-1981 pmid=6947258
		+	#Jacob-JMB-1961 pmid=13718526
		+	#Ptashne-Genetic-Switch isbn=0879697164
		+	</biblio>
		+
		+	==Contact==
		+	*Who has experience with this protocol?
		+
		+	or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].
		+
		+	<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->
		+
		+	<!-- Move the relevant categories above this line to tag your protocol with the label
		+	[[Category:Protocol]]
		+
		+	[[Category:Needs attention]]
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		+	[[Category:In vitro]]
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		+	[[Category:In vivo]]
		+
		+	[[Category:In silico]]
		+
		+	[[Category:DNA]]
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		+	[[Category:RNA]]
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		+	[[Category:Protein]]
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		+	[[Category:Chemical]]
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		+	[[Category:Escherichia coli]]
		+
		+	[[Category:Yeast]]
		+	-->