Mathies:Valves

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{{protocols group|Microfluidics|2=
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<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL!  -->
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{{#dpl:category=Microfluidics|category=Protocol}}
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==Overview==
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This protocol includes all the parts to build the Mathies group Valves and Control System.
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==Materials==
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'''Interested in posting a protocol on OpenWetWare?  Here is a template to help you do so.''' 
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[http://www.stockwell.com/newstuff/newpdfs/500_solid_silicone/rogers_bisco_ht-6000_series_comparitive_chart.pdf PDMS Supply and properties]
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'''Click the view source tab and copy everything below this line. Paste it into your new protocol page. Then replace the text in this page with your own protocol. Feel free to add or delete sections as appropriate.'''
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[http://www.rogerscorporation.com/hpf/IL/PDF/180-076%20HT-6240%20Liquid%20Rubber%20Sheeting.pdf PDMS information]
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[[Category:Template]]
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<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL!  -->
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==Overview==
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==Sample Parts List==
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Replace this sentence with a brief description of the protocol and its goal.
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3.5" x 2.7" miniboard of '''PCB Board''' from www.expresspcb.com
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==Materials==
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You can download their design software, and submit through the software to get a design file.
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List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.
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*supply 1 (i.e. tubes of a certain size? spreaders?)
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Here's the image of PCB circuit board design:
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*reagent 1
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*X &mu;L reagent 2
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**component A (reagent 2 is made up of multiple components)
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**component B
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*equipment 1
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*equipment 2
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==Procedure==
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[[Image:pcbimage1.jpg]]
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#Step 1
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#Step 2
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#*Step 2 has some additional information that goes with it.  i.e. Keep at 4&deg;C.
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#Step 3
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##Step 3 has multiple sub-steps within it.
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##Enumerate each of those.
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==Notes==
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[[Image:pcbimage2.jpg]]
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Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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#List troubleshooting tips here. 
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#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
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#Anecdotal observations that might be of use to others can also be posted here. 
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Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
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[[Image:pcbimage3.jpg]]
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'''Pump''' from Nor-Cal Controls (408)727-5756 www.norcal4air.com
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12V Thomas 7011 Diaphragm Pump
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'''Solenoids''' from Teco Pneumatic (925)426-8500 www.tecopneumatic.com
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8 port manifold for solenoids (need three for 24 lines)
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5V DC Solenoid Valve for vacuum (as many lines as you need, 330 mA each)
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M3 Barbed fitting to 1/8" tubing (as many lines as you need)
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'''Electronics''' from Jameco (800)831-4242 www.jameco.com
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5V@12A and 12V@5A switching AC to DC power supply
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3 pin connector housing (for 5V power connection to board, bags of 30, only need a couple)
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8 pin connector housing (for solenoid connections to board, bags of 30, only need a couple)
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16 pin header straight (for TTL connection to PCB board)
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16 pin connector (attaches to ribbon and fits in header)
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Fuse resettable, 3.0 A-H 6.0 A-T (you may want to change the fuses depending on the number of solenoids and thpump used)
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18 pin low profile IC socket
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Darlington chip ULN2803A
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2 pin connector housing (for 12V power connection to board)
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0.100" Male Breakaway Headers straight single row
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0.100" Male Breakaway Headers straight double row
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0.000" Female crimp pin (fits in the connector housings after crimping on end of wire)
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Ribbon cable (50 line)
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'''Control Cards''' from National Instruments
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NI-PCI-6503 24 line digital I/O
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==Sample Labview Program for Pumping==
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Pneumatic pumps are controlled by Labview programs. Here's a sample pump program (zip file).
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[http://www.cchem.berkeley.edu/ramgrp/protocol/Pump.zip Pump.zip]
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==Assembling Valves Systems==
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[[Image:valvesassembly_1.jpg]]
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==Valve Operating Animations==
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[[Image:pumpaction1.jpg]]
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[[Image:pumpaction2.jpg]]
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[[Image:pumpaction3.jpg]]
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To see the pumping sequence in video, click [http://www.cchem.berkeley.edu/ramgrp/protocol/pumpanimation.gif here].
==References==
==References==
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'''Relevant papers and books'''
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<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. -->
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Papers related to Valves
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<biblio>
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#Goldbeter-PNAS-1981 pmid=6947258
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[http://www.cchem.berkeley.edu/ramgrp/protocol/PDMSvalve.pdf 1 Original PDMS valve paper]
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#Jacob-JMB-1961 pmid=13718526
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[http://www.cchem.berkeley.edu/ramgrp/protocol/Latching.pdf 2 Latching valve paper]
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#Ptashne-Genetic-Switch isbn=0879697164
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[http://www.cchem.berkeley.edu/ramgrp/protocol/Computing.pdf 3 Computing paper]
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</biblio>
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==Contact==
==Contact==
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*Who has experience with this protocol?
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*'''[[User:Eric Chu|Eric Chu]] 22:10, 22 July 2009 (PDT)''':
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*'''[[User:Samantha Cronier|Samantha Cronier]] 19:25, 9 April 2009 (EDT)''':
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].  
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].  
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[[Category:Protocol]]
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[[Category:Microfluidics]]
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[[Category:Protocol]]
[[Category:Protocol]]

Current revision



Contents

Overview

This protocol includes all the parts to build the Mathies group Valves and Control System.

Materials

PDMS Supply and properties

PDMS information

Sample Parts List

3.5" x 2.7" miniboard of PCB Board from www.expresspcb.com

You can download their design software, and submit through the software to get a design file.

Here's the image of PCB circuit board design:

Image:pcbimage1.jpg

Image:pcbimage2.jpg

Image:pcbimage3.jpg


Pump from Nor-Cal Controls (408)727-5756 www.norcal4air.com

12V Thomas 7011 Diaphragm Pump


Solenoids from Teco Pneumatic (925)426-8500 www.tecopneumatic.com

8 port manifold for solenoids (need three for 24 lines)

5V DC Solenoid Valve for vacuum (as many lines as you need, 330 mA each)

M3 Barbed fitting to 1/8" tubing (as many lines as you need)


Electronics from Jameco (800)831-4242 www.jameco.com

5V@12A and 12V@5A switching AC to DC power supply

3 pin connector housing (for 5V power connection to board, bags of 30, only need a couple)

8 pin connector housing (for solenoid connections to board, bags of 30, only need a couple)

16 pin header straight (for TTL connection to PCB board)

16 pin connector (attaches to ribbon and fits in header)

Fuse resettable, 3.0 A-H 6.0 A-T (you may want to change the fuses depending on the number of solenoids and thpump used)

18 pin low profile IC socket

Darlington chip ULN2803A

2 pin connector housing (for 12V power connection to board)

0.100" Male Breakaway Headers straight single row

0.100" Male Breakaway Headers straight double row

0.000" Female crimp pin (fits in the connector housings after crimping on end of wire)

Ribbon cable (50 line)


Control Cards from National Instruments

NI-PCI-6503 24 line digital I/O


Sample Labview Program for Pumping

Pneumatic pumps are controlled by Labview programs. Here's a sample pump program (zip file).

Pump.zip

Assembling Valves Systems

Image:valvesassembly_1.jpg

Valve Operating Animations

Image:pumpaction1.jpg Image:pumpaction2.jpg Image:pumpaction3.jpg

To see the pumping sequence in video, click here.

References

Papers related to Valves

1 Original PDMS valve paper 2 Latching valve paper 3 Computing paper


Contact

or instead, discuss this protocol.

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