http://openwetware.org/index.php?title=Mathies:Separation_Buffer&feed=atom&action=history 2013-05-23T15:06:05Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Mathies:Separation_Buffer&diff=327078&oldid=prev 2009-07-23T04:59:48Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 04:59, 23 July 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 3:</td> <td colspan="2" class="diff-lineno">Line 3:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL!&nbsp; --&gt;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL!&nbsp; --&gt;</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==10X TTE==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==10X TTE==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>10X Tris TAPS EDTA (TTE) solution contains:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>10X Tris TAPS EDTA (TTE) solution contains:</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># 500 mM Tris-base (Bio-Rad, P/N 1610719)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># 500 mM Tris-base (Bio-Rad, P/N 1610719)</div></td></tr> <!-- diff generator: internal 2013-05-23 15:06:05 --> </table>

Eric Chu http://openwetware.org/index.php?title=Mathies:Separation_Buffer&diff=327077&oldid=prev 2009-07-23T04:59:18Z

<p>New page: <a href="/wiki/Category:Protocol" title="Category:Protocol">Category:Protocol</a> <a href="/wiki/Category:Microfluidics" title="Category:Microfluidics">Category:Microfluidics</a> &lt;!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! --&gt; ==10X TTE== 10X Tris TAPS EDTA (TTE) solution contains: # 500 mM Tris...</p> <p><b>New page</b></p><div>[[Category:Protocol]]<br /> [[Category:Microfluidics]]<br /> <br /> &lt;!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! --&gt;<br /> <br /> <br /> ==10X TTE==<br /> <br /> 10X Tris TAPS EDTA (TTE) solution contains:<br /> <br /> <br /> # 500 mM Tris-base (Bio-Rad, P/N 1610719)<br /> <br /> # 500 mM TAPS acid (Sigma, P/N T5130)<br /> <br /> # 10 mM Na2EDTA (Sigma, P/N ED2SS)<br /> <br /> '''To prepare 1 liter of 10X TTE:'''<br /> <br /> 1. Combine the following reagents in a 1-L beaker:<br /> <br /> Tris - 60.55 g<br /> <br /> TAPS acid - 121.65 g<br /> <br /> Na2EDTA - 3.72 g<br /> <br /> Deionized water - q.s. to l L<br /> <br /> 2. Mix well.<br /> <br /> 3. Check the pH of the 10X TTE.<br /> <br /> The pH should be 8.3+/-0.3. If the pH is not in this range, then discard the buffer.<br /> <br /> Store 10X TTE stock solution at 4 degree centigrate.<br /> <br /> '''!WARNING! CHEMICAL HAZARD.''' Tris TAPS EDTA (TTE). May cause eye, skin, and respiratory tract irritation. May be harmful if swallowed. Consult the manufacturer's MSDSs for additional information. Wear splash-resistant safety goggles and appropriate chemical-resistant clothing and gloves.<br /> <br /> ==Contact==<br /> *'''[[User:Eric Chu|Eric Chu]] 22:00, 22 July 2009 (PDT)''':<br /> <br /> or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. <br /> <br /> &lt;!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. --&gt;<br /> <br /> [[Category:Protocol]]<br /> [[Category:Microfluidics]]<br /> <br /> <br /> &lt;!-- Move the relevant categories above this line to tag your protocol with the label<br /> [[Category:Protocol]]<br /> <br /> [[Category:Needs attention]]<br /> <br /> [[Category:In vitro]]<br /> <br /> [[Category:In vivo]]<br /> <br /> [[Category:In silico]]<br /> <br /> [[Category:DNA]]<br /> <br /> [[Category:RNA]]<br /> <br /> [[Category:Protein]]<br /> <br /> [[Category:Chemical]]<br /> <br /> [[Category:Escherichia coli]]<br /> <br /> [[Category:Yeast]]<br /> --&gt;</div>

Eric Chu