http://openwetware.org/index.php?title=Mathies:Dynamic_Coating&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Sat, 18 May 2013 12:52:44 GMT http://openwetware.org/index.php?title=Mathies:Dynamic_Coating&diff=327067&oldid=prev <p>New page: <a href="/wiki/Category:Protocol" title="Category:Protocol">Category:Protocol</a> <a href="/wiki/Category:Microfluidics" title="Category:Microfluidics">Category:Microfluidics</a> &lt;!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! --&gt; ==Coating Procedure Using polyDuramide== polyDuramind (MW~ 2,000,000 g/...</p> <p><b>New page</b></p><div>[[Category:Protocol]]<br /> [[Category:Microfluidics]]<br /> <br /> &lt;!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! --&gt;<br /> <br /> <br /> ==Coating Procedure Using polyDuramide==<br /> <br /> polyDuramind (MW~ 2,000,000 g/mole) is the trade name for poly hydroxyethyleacrylamide (pHEA)<br /> <br /> ==Coating Solution Preparation==<br /> <br /> The dynamic coating solution is prepared at a concentration of 0.25% (wt%) in deionized water.<br /> <br /> Ex. To prepare 20 mL of polyDuramide solution from lyopholized sample:<br /> '''Dissolve 50 mg of polyDuramide in 20 mL of water'''<br /> <br /> ==PolyDuramide Derivitization Procedure==<br /> <br /> (1) Rinse channel with DI water<br /> <br /> (2) Remove water<br /> <br /> (3) Fill channel with 1 M HCl, let stand for 15 minutes<br /> <br /> (4) Remove HCl<br /> <br /> (5) Rinse channel with DI water<br /> <br /> (6) Remove water<br /> <br /> (7) Fill channel with coating solution, let stand for 15 minutes<br /> <br /> (8) Remove coating solution. Chip should be coated.<br /> <br /> If desired, rinse channel with DI water followed with acetonitrile. Dry channel and chip is ready to use.<br /> <br /> ==Coating Storage==<br /> <br /> This coating is very stable. However, when not in use, it would be best to store at ~ 4 °C. <br /> <br /> ==Contact==<br /> *'''[[User:Eric Chu|Eric Chu]] 22:20, 22 July 2009 (PDT)''':<br /> <br /> or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. <br /> <br /> &lt;!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. --&gt;<br /> <br /> [[Category:Protocol]]<br /> [[Category:Microfluidics]]<br /> <br /> <br /> &lt;!-- Move the relevant categories above this line to tag your protocol with the label<br /> [[Category:Protocol]]<br /> <br /> [[Category:Needs attention]]<br /> <br /> [[Category:In vitro]]<br /> <br /> [[Category:In vivo]]<br /> <br /> [[Category:In silico]]<br /> <br /> [[Category:DNA]]<br /> <br /> [[Category:RNA]]<br /> <br /> [[Category:Protein]]<br /> <br /> [[Category:Chemical]]<br /> <br /> [[Category:Escherichia coli]]<br /> <br /> [[Category:Yeast]]<br /> --&gt;</div> Thu, 23 Jul 2009 04:30:13 GMT Eric Chu http://openwetware.org/wiki/Talk:Mathies:Dynamic_Coating