Materials

Media

Defined Media

• AB medium
• M9 medium
• M9 salts
• Neidhardt EZ Rich Defined
• SC
• SD

Standard Media

• 7H9
• DYT
• GYT
• LB
• Mesoplama florum:Culture media
• SOB
• SOC
• SR
• T broth
• Terrific Broth
• YEPD Liquid
• YNB media
• YPD
• YT

Antibiotics

• Ampicillin
• Carbenicillin
• Chloramphenicol
• ClonNat
• Erythromycin
• G418
• Gentamicin
• Kanamycin
• Nalidixic Acid
• Neomycin
• Phleomycin
• Rifampicin
• Spectinomycin
• Springer Lab: Antibiotics
• Streptomyces:Other Bits/Antibiotic and Resistance Marker Information
• Streptomycin
• Tetracycline
• Trimethoprim

Enzymes

Phosphatases

• Antarctic Phosphatase

DNA polymerases

• Pfu
• Pfx50
• Phusion
• Reverse Transcriptase
• T4 DNA Polymerase
• Terminal Transferase

DNA ligases

• Thermostable DNA ligase

RNA Polymerases

• Escherichia coli RNA polymerase

Restriction enzymes

• DpnI
• EcoRI
• MfeI
• NheI
• NsiI
• PstI
• SpeI
• XbaI
• XmaI

Other

• Lysozyme

Proteases

• Proteinase K

Buffers

• B-Per II
• CAPS
• Destain
• EDTA
• HEPES
• Kafatos:10x PBS
• Kafatos:P1
• Kafatos:P2
• Kafatos:P3
• Killing Buffer
• MES
• MOPS
• NEB buffers
• PBS
• PBST
• PIPES
• Phosphate buffer
• Qiagen Buffers
• S30 buffer
• SDS sample buffer
• SSC
• Silver: 50x TAE
• Succinic acid
• TAE
• TB
• TBE
• TBS
• TBST
• TE
• TSS
• Tris

Acids and bases

• 5-Fluoroorotic acid
• Acid concentration chart
• Glycine
• Hydrochloric acid
• Sodium acetate
• Sodium hydroxide

Other common chemicals

• 1 normal acid or base (1N)
• 2-methylbutane (isopentane)
• 3-Aminotriazole
• AEBSF
• ATc
• Acridine Orange
• Arabinose
• Bromodeoxyuridine (BrdU)
• Cell and tissue lysis hub
• Cresol Red
• DEPC
• DMF
• DMPC
• EDTA
• EGTA
• Ethidium Bromide
• Ficoll
• Glucose
• Glutaraldehyde (Pentanedial)
• Glycerol
• IPTG
• Isoamyl alcohol
• ONP
• ONPF
• ONPG
• PMSF
• Pefabloc
• Phenol
• Raffinose
• Spermine
• X-Gal

Fixatives

• Formaldehyde
• Glutaraldehyde (Pentanedial)
• Polymethanal (paraformaldehyde)

Reporters

Dyes and stains

• 7-AAD
• Acridine Orange
• Annexin-V
• Carboxyfluorescein Diacetate
• DAPI
• Fluorescein diacetate
• Hoechst 33342
• Propidium iodide
• SYBR Gold
• SYBR Green I
• SYBR Green II
• Sybr Safe
• SYTO

Fluorescent proteins

• CFP
• Emerald
• GFP
• MRFP1
• YFP

Enzymes

• Beta-galactosidase
• Beta-lactamase

Electrophoresis

Agarose gel electrophoresis

• Agarose gel loading buffer
• Bromophenol blue
• Cresol Red
• DNA ladder
• Ethidium Bromide
• Kafatos:DNA 1kbplus ladder
• SB
• Silver: 50x TAE
• Sybr Safe
• TAE
• TBE
• Xylene cyanol

PAGE

• Coomassie stain
• Destain
• PLS
• SDS-PAGE

Miscellaneous

• DNTPs
• Materials/Additional Categories Needed
• Materials/Search
• Materials/Template
• Protein purification tags
• ProteinA Sepharose Beads

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