Materials

Media

Defined Media

• AB medium
• M9 medium
• M9 salts
• Neidhardt EZ Rich Defined
• SC
• SD

Standard Media

• 7H9
• DYT
• GYT
• LB
• Mesoplama florum:Culture media
• SOB
• SOC
• SR
• T broth
• Terrific Broth
• YEPD Liquid
• YNB media
• YPD
• YT

Cell Culture Media

• DMEM
• RPMI
• complete DMEM

Antibiotics

• Ampicillin
• Carbenicillin
• Chloramphenicol
• ClonNat
• Erythromycin
• G418
• Gentamicin
• Kanamycin
• Nalidixic Acid
• Neomycin
• Phleomycin
• Rifampicin
• Spectinomycin
• Springer Lab: Antibiotics
• Streptomyces:Other Bits/Antibiotic and Resistance Marker Information
• Streptomycin
• Tetracycline
• Trimethoprim

Enzymes

Phosphatases

• Antarctic Phosphatase

DNA polymerases

• Pfu
• Pfx50
• Phusion
• Reverse Transcriptase
• T4 DNA Polymerase
• Terminal Transferase

RNA Polymerases

• Escherichia coli RNA polymerase

Restriction enzymes

• DpnI
• EcoRI
• MfeI
• NheI
• NsiI
• PstI
• SpeI
• XbaI
• XmaI

Buffers

• ACK lysis buffer
• B-Per II - lysis buffer
• HEPES
• MES
• NEB buffers
• PIPES
• PBS
• SDS sample buffer
• SSC buffer
• TB buffer
• TE buffer
• Tris
• TSS
• Phosphate buffer

Acids and bases

• Acid concentration chart
• 5-Fluoroorotic acid
• Sodium hydroxide

Other common chemicals

• 1 normal acid or base (1N)
• 2-methylbutane (isopentane)
• 3-Aminotriazole
• AEBSF
• ATc
• Acridine Orange
• Arabinose
• Bromodeoxyuridine (BrdU)
• Cell and tissue lysis hub
• Cresol Red
• DEPC
• DMF
• DMPC
• EDTA
• EGTA
• Ethidium Bromide
• Ficoll
• Glucose
• Glutaraldehyde (Pentanedial)
• Glycerol
• IPTG
• Isoamyl alcohol
• ONP
• ONPF
• ONPG
• PMSF
• Pefabloc
• Phenol
• Raffinose
• Spermine
• X-Gal

Fixatives

• polymethanal (paraformaldehyde)
• glutaraldehyde

Reporters

Dyes and stains

• Acridine Orange
• Carboxyfluorescein Diacetate
• DAPI
• Fluorescein diacetate
• Hoechst 33342
• Propidium iodide
• SYBR Gold
• SYBR Green I
• SYBR Green II
• Sybr Safe
• SYTO

Fluorescent proteins

• CFP
• Emerald-GFP
• GFP
• mRFP1
• YFP

Enzymes

• Beta-galactosidase
• Beta-lactamase

Electrophoresis

Agarose gel electrophoresis

• Agarose gel loading buffer
• Bromophenol blue
• Cresol Red
• DNA ladder
• Ethidium Bromide
• Kafatos:DNA 1kbplus ladder
• SB
• Silver: 50x TAE
• Sybr Safe
• TAE
• TBE
• Xylene cyanol

PAGE

• Coomassie stain
• Destain
• PLS
• SDS-PAGE

• SDS-PAGE
• Destain
• Coomassie stain
• PLS

Miscellaneous

• dNTPs
• ProteinA_Sepharose Beads

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