Marie-Emmanuelle Kerros
From OpenWetWare
HOW TO ... MAKE PCR REAGENTS
Rutgers, October 2004
1- 5x Tris Boric acid EDTA buffer
For 2 liters: - TRIS base (Boehringer Mannheim 1814273): 108 g - Boric acid electrophoresis grade (FisherBiotech BP168-500): 55 g - 0.5 M EDTA (pH 8.0): 40 ml (or 9.3 g Na2EDTA) - adjust to pH 8.3 - H2O to 2 liters
2- EDTA 0.5 M pH = 8.0
- EDTA: 18.61 g
- NaOH: 2.0 g
- H2O: 80 ml
- dissolve on a magnetic stirrer
- adjust to pH = 8.0 with NaOH 10N and 1N
- H2O to 100 ml