Marek: Freeze-down/Thaw

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Contents

Overview

This short protocol describes how to freeze down and thaw (or bring up) mammalian cells (e.g. HeLa, KEK293, CHO, Jurkat T cells).

Materials

List reagents, supplies and equipment necessary to perform the protocol here.

  • 10-50 ml centrifuge tube
  • DMSO, Tissue culture (TC)-tested (e.g. Sigma, D2650)
  • Freeze-down medium
    • 6% DMSO
    • Cell culture medium
  • Basic TC centriguge (0 - 2500 rpm)
  • Freeze down box (this could be simply made from a small styropore box and cotton or from two styropore racks from 15-ml centrifuge tubes )

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.


Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.

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