Marek: Freeze-down/Thaw

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(Freezing down mammalian cells)
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==Creating a New Protocol==
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==Overview==
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* [[OpenWetWare:Searching | Search]] for your protocol or page to verify that is not already in existence
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* Click on "create this page"
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This short protocol describes how to freeze down and thaw (or bring up) mammalian cells (e.g. HeLa, KEK293, CHO, Jurkat T cells).
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* View and copy the source from the [[Protocols/Template|'''protocols template''']] in to your new page and begin editing.
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==Materials==
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List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.
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*supply 1 (i.e. tubes of a certain size? spreaders?)
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*reagent 1
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*X μL reagent 2
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**component A (reagent 2 is made up of multiple components)
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**component B
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*equipment 1
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*equipment 2
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==Procedure==
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#Step 1
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#Step 2
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#*Step 2 has some additional information that goes with it.  i.e. Keep at 4°C.
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#Step 3
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##Step 3 has multiple sub-steps within it.
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##Enumerate each of those.
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==Notes==
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#List troubleshooting tips here. 
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#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
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#Anecdotal observations that might be of use to others can also be posted here. 
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Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
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==References==
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'''Relevant papers and books'''
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<!-- If this protocol has papers or books associated with it, list those references here.  See the [[OpenWetWare:Biblio]] page for more information. -->
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<biblio>
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#Goldbeter-PNAS-1981 pmid=6947258
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#Jacob-JMB-1961 pmid=13718526
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#Ptashne-Genetic-Switch isbn=0879697164
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</biblio>
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==Contact==
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*Who has experience with this protocol?
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or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].
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<!-- You can tag this protocol with various categories.  See the [[Categories]] page for more information. -->
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[[Category:Protocol]]
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[[Category:Needs attention]]  <!--Delete this line once the protocol is complete-->
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<!-- Move the relevant categories above this line to tag your protocol with the label
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[[Category:In vitro]]
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[[Category:In vivo]]
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[[Category:In silico]]
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[[Category:DNA]]
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[[Category:RNA]]
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[[Category:Protein]]
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[[Category:Chemical]]
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[[Category:Escherichia coli]]
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[[Category:Yeast]]
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-->

Revision as of 04:43, 18 May 2007

Contents

Overview

This short protocol describes how to freeze down and thaw (or bring up) mammalian cells (e.g. HeLa, KEK293, CHO, Jurkat T cells).

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

  • supply 1 (i.e. tubes of a certain size? spreaders?)
  • reagent 1
  • X μL reagent 2
    • component A (reagent 2 is made up of multiple components)
    • component B
  • equipment 1
  • equipment 2

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. . pmid:6947258. PubMed HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. . pmid:13718526. PubMed HubMed [Jacob-JMB-1961]
  3. Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164. [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed HubMed

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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