Malaria

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== Plasmids ==
== Plasmids ==
*Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
*Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
-
**pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid
+
**pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid [[file:pCP08.xdna]]
**pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid
**pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid
**pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
**pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
all plasmids are AmpR
all plasmids are AmpR

Revision as of 10:24, 30 August 2006

Live replicating adenovirus malaria vaccine

Plasmids

  • Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
    • pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid file:pCP08.xdna
    • pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid
    • pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid

all plasmids are AmpR

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