Making RNA probes with T7 transcription
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Jakob Suckale
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mRNA sense & antisense probes
Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.
See also
In vitro transcription with T7 RNA polymerase
Primer design
External links
T7 transcription protocol by Jonathan Davies of Harvard Uni (1998)
In vitro transcription basics at Ambion
T7 RNA polymerase - Wikipedia
,
RNA polymerases - Fermentas
]
Categories
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Protocol
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RNA
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In vitro
In vitro
Protocol
RNA
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Jakob Suckale
