Making RNA probes with T7 transcription - Revision history

Jakob Suckale: back to protocols

Jakob Suckale — Sat, 20 Jun 2009 15:33:00 GMT

back to protocols

←Older revision		Revision as of 15:33, 20 June 2009
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	== mRNA sense & antisense probes ==		== mRNA sense & antisense probes ==
	[[Image:Antisense_probes_and_mRNA.png\|thumb\|left\|500px\|Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.]]		[[Image:Antisense_probes_and_mRNA.png\|thumb\|left\|500px\|Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.]]

Jakob Suckale — Sat, 20 Jun 2009 15:29:57 GMT

line breaks

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	== mRNA sense & antisense probes ==		== mRNA sense & antisense probes ==
	[[Image:Antisense_probes_and_mRNA.png\|thumb\|left\|500px\|Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.]]		[[Image:Antisense_probes_and_mRNA.png\|thumb\|left\|500px\|Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.]]
		+	<br clear="all" />

	== In vitro T7 transcription of an RNA probe ==		== In vitro T7 transcription of an RNA probe ==
	[[Image:T7 primer design for RNA antisense probes.png\|thumb\|left\|500px\|Designing primers for T7 RNA synthesis]]		[[Image:T7 primer design for RNA antisense probes.png\|thumb\|left\|500px\|Designing primers for T7 RNA synthesis]]
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	# designing a suitable primer combining the T7 promoter sequence and a sequence specific part		# designing a suitable primer combining the T7 promoter sequence and a sequence specific part
	# attaching a T7 promoter via PCR		# attaching a T7 promoter via PCR
	# in vitro transcription using T7 polymerase and the PCR product as a template		# in vitro transcription using T7 polymerase and the PCR product as a template
		+

	== See also ==		== See also ==

Jakob Suckale — Sat, 20 Jun 2009 15:27:19 GMT

In vitro T7 transcription of an RNA probe

←Older revision		Revision as of 15:27, 20 June 2009
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	== mRNA sense & antisense probes ==		== mRNA sense & antisense probes ==
	[[Image:Antisense_probes_and_mRNA.png\|thumb\|left\|500px\|Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.]]		[[Image:Antisense_probes_and_mRNA.png\|thumb\|left\|500px\|Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.]]
		+
		+	== In vitro T7 transcription of an RNA probe ==
		+	[[Image:T7 primer design for RNA antisense probes.png\|thumb\|left\|500px\|Designing primers for T7 RNA synthesis]]
		+	# designing a suitable primer combining the T7 promoter sequence and a sequence specific part
		+	# attaching a T7 promoter via PCR
		+	# in vitro transcription using T7 polymerase and the PCR product as a template

	== See also ==		== See also ==

Jakob Suckale — Sat, 20 Jun 2009 15:20:08 GMT

mRNA sense, probe antisense

←Older revision		Revision as of 15:20, 20 June 2009
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		+	== mRNA sense & antisense probes ==
		+	[[Image:Antisense_probes_and_mRNA.png\|thumb\|left\|500px\|Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.]]
		+
	== See also ==		== See also ==
	* [[In vitro transcription with T7 RNA polymerase]]		* [[In vitro transcription with T7 RNA polymerase]]

Jakob Suckale — Sat, 20 Jun 2009 15:08:14 GMT

←Older revision		Revision as of 15:08, 20 June 2009
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-
	== See also ==		== See also ==
	* [[In vitro transcription with T7 RNA polymerase]]		* [[In vitro transcription with T7 RNA polymerase]]
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	* [http://genetics.mgh.harvard.edu/szostakweb/protocols/transcription/index.html T7 transcription protocol by Jonathan Davies of Harvard Uni (1998)]		* [http://genetics.mgh.harvard.edu/szostakweb/protocols/transcription/index.html T7 transcription protocol by Jonathan Davies of Harvard Uni (1998)]
	* [http://www.ambion.com/techlib/basics/transcription/index.html In vitro transcription basics at Ambion]		* [http://www.ambion.com/techlib/basics/transcription/index.html In vitro transcription basics at Ambion]
-	* [http://en.wikipedia.org/wiki/T7_RNA_polymerase T7 RNA polymerase - Wikipedia]	+	* [http://en.wikipedia.org/wiki/T7_RNA_polymerase T7 RNA polymerase - Wikipedia], [http://www.fermentas.com/catalog/modifyingenzymes/rnapolymerases.htm RNA polymerases - Fermentas]]

	[[Category:Protocol]]		[[Category:Protocol]]
	[[Category:RNA]]		[[Category:RNA]]
	[[Category:In vitro]]		[[Category:In vitro]]

Jakob Suckale — Sat, 20 Jun 2009 15:06:11 GMT

Jakob Suckale — Sat, 20 Jun 2009 15:04:54 GMT

external links

←Older revision		Revision as of 15:04, 20 June 2009
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		+
		+	== External links ==
		+	* [http://genetics.mgh.harvard.edu/szostakweb/protocols/transcription/index.html T7 transcription protocol by Jonathan Davies of Harvard Uni (1998)]
		+	* [http://www.ambion.com/techlib/basics/transcription/index.html In vitro transcription basics at Ambion]
		+	* [http://en.wikipedia.org/wiki/T7_RNA_polymerase T7 RNA polymerase - Wikipedia]

	[[Category:Protocol]]		[[Category:Protocol]]
	[[Category:RNA]]		[[Category:RNA]]
	[[Category:In vitro]]		[[Category:In vitro]]

Jakob Suckale — Sat, 20 Jun 2009 15:03:13 GMT

*** page created ***

New page

[[Category:Protocol]]
[[Category:RNA]]
[[Category:In vitro]]

←Older revision		Revision as of 15:06, 20 June 2009
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		+	== See also ==
		+	* [[In vitro transcription with T7 RNA polymerase]]
		+	* [[Primer design]]

	== External links ==		== External links ==