Magni:Protocols/ph: Difference between revisions
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**glycerol and | **glycerol and | ||
**M9 salts. | **M9 salts. | ||
*Dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required); | |||
* ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections! | |||
* wash the pH probe with deionized water and store it in KCl 3M. | |||
* Autoclave the mix in a bottle. | * Autoclave the mix in a bottle. | ||
* Equilibrate at room temperature and add the remaining ingredients (casamino acids, thiamine, CaCl2, MgSO4). | * Equilibrate at room temperature and add the remaining ingredients (casamino acids, thiamine, CaCl2, MgSO4). | ||
</div> | </div> | ||
Revision as of 08:44, 18 April 2013
pH adjustment for LB medium
- Before autoclaving the LB medium:
- dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
- ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
- wash the pH probe with deionized water and store it in KCl 3M.
- Autoclave the medium
pH adjustment for M9 medium
- According to the M9 medium preparation protocol, mix:
- water,
- glycerol and
- M9 salts.
- Dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
- ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
- wash the pH probe with deionized water and store it in KCl 3M.
- Autoclave the mix in a bottle.
- Equilibrate at room temperature and add the remaining ingredients (casamino acids, thiamine, CaCl2, MgSO4).
