Macroscope: Difference between revisions

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=== notes ===
=== notes ===
* Fluorescence shading correction. Divide images by a flat field standard
*
=== improvements ===
=== improvements ===
* Better darkfield illumination
* 3D imaging of fluorescence in transparent volumes using sheet illumination (laser, cylindrical lens, )
== Similar Tech ==
* Chait R, Shrestha S, Shah AK, Michel J-B, Kishony R (2010) A Differential Drug Screen for Compounds That Select Against Antibiotic Resistance. PLoS ONE 5(12): e15179. doi:10.1371/journal.pone.0015179
* ? Ian Chin-Sang1 and Weiwei Zhong Using LEDs as a low-cost source to detect GFP and DsRED Worm Breeder's Gazette
Department of Biology, Queen’s University, Kingston, ON, Canada, 2Department of Biochemistry and Cell Biology, Rice University, Houston TX


== refs, worms p1 ==





Revision as of 09:51, 9 December 2011

In the beginning, there was Disco!

coming soon ...


Macroscope:

what

why

capabilites (current and potential)

To build:

parts list and sources

basic structure

custom parts (cam and filter mounts)

camera

filters and leds - chroma...clearance...mention gel filters...

control box

software (link to canon SDK req; starlight express drivers, switch&sense)

etc

Thoughts:

notes

  • Fluorescence shading correction. Divide images by a flat field standard

improvements

  • Better darkfield illumination
  • 3D imaging of fluorescence in transparent volumes using sheet illumination (laser, cylindrical lens, )


Similar Tech

  • Chait R, Shrestha S, Shah AK, Michel J-B, Kishony R (2010) A Differential Drug Screen for Compounds That Select Against Antibiotic Resistance. PLoS ONE 5(12): e15179. doi:10.1371/journal.pone.0015179
  • ? Ian Chin-Sang1 and Weiwei Zhong Using LEDs as a low-cost source to detect GFP and DsRED Worm Breeder's Gazette

Department of Biology, Queen’s University, Kingston, ON, Canada, 2Department of Biochemistry and Cell Biology, Rice University, Houston TX