MOPS
From OpenWetWare
MOPS is the common name for the buffering compound in MOPS buffer. MOPS stands for 3-(N-morpholino) propanesulfonic acid and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. HEPES is a chemically similar pH buffering compound.
Recipe for 10x MOPS buffer
- 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
- 10.9g Sodium Acetate, 3- hydrate
- 3.7g EDTA, sodium salt
- add 800ml of nuclease free distilled water; mix to dissolve
- adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
- fill to the final volume of 1000 ml
- filter sterilise
- store at room temperature
- protect from light; do not use if the solution appears yellow
Some OWW protocols which use MOPS
- Jacobs:Protocol RNA Agarose Gel
- Knight:NuPAGE electrophoresis
- Sauer:bis-Tris SDS-PAGE, the very best
