MOPS: Difference between revisions
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(Stability of MOPS) |
(Final concentration) |
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* store at room temperature | * store at room temperature | ||
* protect from light; do not use if the solution appears yellow | * protect from light; do not use if the solution appears yellow | ||
==Final concentration of active compounds== | |||
* 400mM MOPS (buffering) | |||
* 100mM NaAc | |||
* 10mM [[EDTA]] (nuclease inhibition by Mg2+ chelation) | |||
==Stability of MOPS== | ==Stability of MOPS== | ||
Revision as of 09:08, 2 March 2009
MOPS is the common name for the buffering compound in MOPS buffer. MOPS stands for 3-(N-morpholino) propanesulfonic acid and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. HEPES is a chemically similar pH buffering compound.
Recipe for 10x MOPS buffer
- 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
- 10.9g Sodium Acetate, 3- hydrate
- 3.7g EDTA, sodium salt
- add 800ml of nuclease free distilled water; mix to dissolve
- adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
- fill to the final volume of 1000 ml
- filter sterilise or autoclave
- store at room temperature
- protect from light; do not use if the solution appears yellow
Final concentration of active compounds
- 400mM MOPS (buffering)
- 100mM NaAc
- 10mM EDTA (nuclease inhibition by Mg2+ chelation)
Stability of MOPS
Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. This will not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [1]
Some OWW protocols which use MOPS
- Jacobs:Protocol RNA Agarose Gel
- Knight:NuPAGE electrophoresis
- Sauer:bis-Tris SDS-PAGE, the very best
