MOPS

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(Some OWW protocols which use MOPS)
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==Recipe for 10x MOPS buffer==
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[[Image:800px-Mops2.JPG|right|200px|thumb|The other mops [http://en.wikipedia.org/wiki/Pug] ]]
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* 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
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'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'', and with a pKa of 7.20, makes a good buffering agent for many biological systems requiring neutral pH. [[HEPES]] is a chemically similar pH buffering compound.  
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* 10.9g Sodium Acetate, 3- hydrate
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* 3.7g EDTA, sodium salt
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==Recipe for 10x MOPS buffer, 1 L==
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[[Image:800px-MOPS.png|right|200px|thumb|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]
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* add 800ml of nuclease free distilled water; mix to dissolve
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* 83.7 g MOPS; MW 209.3 g/mol
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* 13.6 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is only 82 g/mol or 8.2g)
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* 3.7 g EDTA, disodium dihydrate; MW 372.24 g/mol (again check MW of the salt you stock)
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* add 800 ml of nuclease free distilled water; mix to dissolve
* adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
* adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
* fill to the final volume of 1000 ml
* fill to the final volume of 1000 ml
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* filter sterilise
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* filter sterilise or autoclave
* store at room temperature
* store at room temperature
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* protect from light; do not use if the solution appears yellow
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* protect from light; do not use if the solution is dark (yellow is ok)
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==Final concentration in 10x stock==
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* 400 mM MOPS (buffering)
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* 100 mM NaAc
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* 10 mM [[EDTA]] (nuclease inhibition by Mg2+ chelation)
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==Variation==
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Some protocols use a less concentrated MOPS buffer
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* 200 mM MOPS - 41.9 g in 1 L
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* 20 mM NaAc - 2.7 g
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* 10 mM [[EDTA]] - 3.7 g
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==Stability of MOPS==
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Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. Solution will turn yellow but this does not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [http://books.google.de/books?id=h5lGFPz054oC&pg=PA201&dq=farrel+rna+mops&lr=&ei=RAGsSb_8NouYMp7enJIF&hl=en]. Straw coloured buffer is good but do not use darker buffer.
==Some OWW protocols which use MOPS==
==Some OWW protocols which use MOPS==
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* [[Knight:NuPAGE electrophoresis]]
* [[Knight:NuPAGE electrophoresis]]
* [[Sauer:bis-Tris SDS-PAGE, the very best]]
* [[Sauer:bis-Tris SDS-PAGE, the very best]]
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== External links ==
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* [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS]
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* [http://www.fermentas.com/techinfo/electrophoresis/rnaloading.htm#MOPS_ MOPS recipe at Fermentas]
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* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=M5755|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC premade MOPS 10x from Sigma]
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* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8332?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=mops&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT&text_only=true CSH Protocols MOPS] [[Image:Padlock-closed.png]]
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[[Category:Material]] and [[Category:Buffers]]

Current revision

The other mops [1]
The other mops [1]

MOPS is the common name for the buffering compound in MOPS buffer. MOPS stands for 3-(N-morpholino) propanesulfonic acid, and with a pKa of 7.20, makes a good buffering agent for many biological systems requiring neutral pH. HEPES is a chemically similar pH buffering compound.

Contents

Recipe for 10x MOPS buffer, 1 L

chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid
chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid
  • 83.7 g MOPS; MW 209.3 g/mol
  • 13.6 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is only 82 g/mol or 8.2g)
  • 3.7 g EDTA, disodium dihydrate; MW 372.24 g/mol (again check MW of the salt you stock)


  • add 800 ml of nuclease free distilled water; mix to dissolve
  • adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
  • fill to the final volume of 1000 ml


  • filter sterilise or autoclave
  • store at room temperature
  • protect from light; do not use if the solution is dark (yellow is ok)

Final concentration in 10x stock

  • 400 mM MOPS (buffering)
  • 100 mM NaAc
  • 10 mM EDTA (nuclease inhibition by Mg2+ chelation)

Variation

Some protocols use a less concentrated MOPS buffer

  • 200 mM MOPS - 41.9 g in 1 L
  • 20 mM NaAc - 2.7 g
  • 10 mM EDTA - 3.7 g

Stability of MOPS

Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. Solution will turn yellow but this does not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [2]. Straw coloured buffer is good but do not use darker buffer.

Some OWW protocols which use MOPS

External links

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