MOPS

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(Recipe for 10x MOPS buffer: mol weight)
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'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.  
'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.  
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==Recipe for 10x MOPS buffer==
==Recipe for 10x MOPS buffer==
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* 41.2 g 3-(N-morpholino) propanesulfonic acid (MOPS); MW 209.3 g/mol
* 41.2 g 3-(N-morpholino) propanesulfonic acid (MOPS); MW 209.3 g/mol
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* 10.9 g Sodium Acetate, 3- hydrate; MW 136.1 g/mol  
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* 10.9 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is lighter with MW = 82 g/mol)
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: (watch out: NaAc, anhydrous is lighter with MW = 82 g/mol)
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* 3.7 g EDTA, sodium salt; MW 292.2 g/mol
* 3.7 g EDTA, sodium salt; MW 292.2 g/mol
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* protect from light; do not use if the solution appears yellow
* protect from light; do not use if the solution appears yellow
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==Final concentration of active compounds==
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==Final concentration of active compounds in 10x stock==
* 400 mM MOPS (buffering)
* 400 mM MOPS (buffering)
* 100 mM NaAc
* 100 mM NaAc
* 10 mM [[EDTA]] (nuclease inhibition by Mg2+ chelation)
* 10 mM [[EDTA]] (nuclease inhibition by Mg2+ chelation)
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==Stability of MOPS==
==Stability of MOPS==
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Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. This will not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [http://books.google.de/books?id=h5lGFPz054oC&pg=PA201&dq=farrel+rna+mops&lr=&ei=RAGsSb_8NouYMp7enJIF&hl=en]
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Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. Solution will turn yellow but this does not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [http://books.google.de/books?id=h5lGFPz054oC&pg=PA201&dq=farrel+rna+mops&lr=&ei=RAGsSb_8NouYMp7enJIF&hl=en]
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* [[Knight:NuPAGE electrophoresis]]
* [[Knight:NuPAGE electrophoresis]]
* [[Sauer:bis-Tris SDS-PAGE, the very best]]
* [[Sauer:bis-Tris SDS-PAGE, the very best]]
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== External links ==
== External links ==
* [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS]
* [http://en.wikipedia.org/wiki/MOPS Wikipedia MOPS]

Revision as of 12:31, 2 March 2009

The other mops
The other mops

MOPS is the common name for the buffering compound in MOPS buffer. MOPS stands for 3-(N-morpholino) propanesulfonic acid and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. HEPES is a chemically similar pH buffering compound.


Contents

Recipe for 10x MOPS buffer

chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid
chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid
  • 41.2 g 3-(N-morpholino) propanesulfonic acid (MOPS); MW 209.3 g/mol
  • 10.9 g Sodium Acetate, trihydrate; MW 136.1 g/mol (watch out: NaAc, anhydrous is lighter with MW = 82 g/mol)
  • 3.7 g EDTA, sodium salt; MW 292.2 g/mol


  • add 800 ml of nuclease free distilled water; mix to dissolve
  • adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
  • fill to the final volume of 1000 ml


  • filter sterilise or autoclave
  • store at room temperature
  • protect from light; do not use if the solution appears yellow


Final concentration of active compounds in 10x stock

  • 400 mM MOPS (buffering)
  • 100 mM NaAc
  • 10 mM EDTA (nuclease inhibition by Mg2+ chelation)


Stability of MOPS

Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. Solution will turn yellow but this does not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [1]


Some OWW protocols which use MOPS


External links

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