MOPS

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(illustration)
(Stability of MOPS)
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* filter sterilise
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* filter sterilise or autoclave
* store at room temperature
* store at room temperature
* protect from light; do not use if the solution appears yellow
* protect from light; do not use if the solution appears yellow
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 +
==Stability of MOPS==
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Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. This will not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [http://books.google.de/books?id=h5lGFPz054oC&pg=PA201&dq=farrel+rna+mops&lr=&ei=RAGsSb_8NouYMp7enJIF&hl=en]
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==Some OWW protocols which use MOPS==
==Some OWW protocols which use MOPS==

Revision as of 11:56, 2 March 2009

The other mops
The other mops

MOPS is the common name for the buffering compound in MOPS buffer. MOPS stands for 3-(N-morpholino) propanesulfonic acid and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. HEPES is a chemically similar pH buffering compound.

Contents

Recipe for 10x MOPS buffer

chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid
chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid
  • 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
  • 10.9g Sodium Acetate, 3- hydrate
  • 3.7g EDTA, sodium salt


  • add 800ml of nuclease free distilled water; mix to dissolve
  • adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
  • fill to the final volume of 1000 ml


  • filter sterilise or autoclave
  • store at room temperature
  • protect from light; do not use if the solution appears yellow

Stability of MOPS

Contrary to common belief, MOPS is sufficiently heat-stable to be autoclaved. This will not interfere with its buffering capacity. See, for example, Farrell RNA methods, p201 [1]


Some OWW protocols which use MOPS

External links

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