MOPS

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m (Recipe for 10x MOPS buffer)
(Some OWW protocols which use MOPS)
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* store at room temperature
* store at room temperature
* protect from light; do not use if the solution appears yellow
* protect from light; do not use if the solution appears yellow
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 +
==Some OWW protocols which use MOPS==
 +
* [[Jacobs:Protocol RNA Agarose Gel]]
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* [[Knight:NuPAGE electrophoresis]]
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* [[Sauer:bis-Tris SDS-PAGE, the very best]]

Revision as of 10:33, 2 March 2009

Recipe for 10x MOPS buffer

  • 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
  • 10.9g Sodium Acetate, 3- hydrate
  • 3.7g EDTA, sodium salt


  • add 800ml of nuclease free distilled water; mix to dissolve
  • adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
  • fill to the final volume of 1000 ml


  • filter sterilise
  • store at room temperature
  • protect from light; do not use if the solution appears yellow

Some OWW protocols which use MOPS

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