MOPS

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(Recipe for 10x MOPS buffer)
m (Recipe for 10x MOPS buffer)
Line 4: Line 4:
* 10.9g Sodium Acetate, 3- hydrate
* 10.9g Sodium Acetate, 3- hydrate
* 3.7g EDTA, sodium salt
* 3.7g EDTA, sodium salt
 +
* add 800ml of nuclease free distilled water; mix to dissolve
* add 800ml of nuclease free distilled water; mix to dissolve
* adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
* adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
* fill to the final volume of 1000 ml
* fill to the final volume of 1000 ml
 +
* filter sterilise
* filter sterilise
* store at room temperature
* store at room temperature
* protect from light; do not use if the solution appears yellow
* protect from light; do not use if the solution appears yellow

Revision as of 11:31, 2 March 2009

Recipe for 10x MOPS buffer

  • 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
  • 10.9g Sodium Acetate, 3- hydrate
  • 3.7g EDTA, sodium salt


  • add 800ml of nuclease free distilled water; mix to dissolve
  • adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
  • fill to the final volume of 1000 ml


  • filter sterilise
  • store at room temperature
  • protect from light; do not use if the solution appears yellow
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