MEG: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 19: | Line 19: | ||
===Reference=== | ===Reference=== | ||
This work was done by [[Caitlin Conboy]] and [[ | This work was done by [[Caitlin Conboy]] and [[Jennifer Braff]]. Thorough documentation to come... |
Revision as of 15:21, 29 November 2005
Molecules of Equivalent GFP (MEG)
Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot.
The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted.
Standard curve
[Fluorescence intensity] = 0.0025*[GFP copies/cell]-14.416
R^2 = 0.9684
FP-0852-2 MEG
From the standard curve above, FP-0852-2 beads represent ~370,000 MEG (+/-40%)
Reference
This work was done by Caitlin Conboy and Jennifer Braff. Thorough documentation to come...