MEG: Difference between revisions
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==Molecules of Equivalent GFP (MEG)== | ==Molecules of Equivalent GFP (MEG)== | ||
[[Image:Cconboy-Flow Calibration.png|200px]] | |||
[Image:Cconboy-Flow Calibration.png|200px] | |||
Fig.1. Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quantitative western blot. The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted. | |||
The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted. | |||
===Standard curve=== | ===Standard curve=== | ||
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===Reference=== | ===Reference=== | ||
This work was done by [[Caitlin Conboy]] and [[ | This work was done by [[Caitlin Conboy]] and [[Jennifer Braff]]. Thorough documentation to come... | ||
Latest revision as of 18:36, 29 May 2013
Molecules of Equivalent GFP (MEG)
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[Image:Cconboy-Flow Calibration.png|200px]
Fig.1. Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quantitative western blot. The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted.
Standard curve
[Fluorescence intensity] = 0.0025*[GFP copies/cell]-14.416
R^2 = 0.9684
FP-0852-2 MEG
From the standard curve above, FP-0852-2 beads represent ~370,000 MEG (+/-40%)
Reference
This work was done by Caitlin Conboy and Jennifer Braff. Thorough documentation to come...
