MEG: Difference between revisions
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==Molecules of Equivalent GFP (MEG)== | ==Molecules of Equivalent GFP (MEG)== | ||
Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot. | Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot. | ||
[[Image:FP-0852-2 MEG calibration.pdf]] | [[Image:FP-0852-2 MEG calibration.pdf]] | ||
The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted. | |||
===Standard curve=== | |||
[Fluorescence intensity] = 0.0025*[GFP copies/cell]-14.416 | |||
R^2 = 0.9684 | |||
===FP-0852-2 MEG=== | |||
From the standard curve above, FP-0852-2 beads represent ~370,000 MEG (+/-40%) | |||
Revision as of 15:18, 29 November 2005
Molecules of Equivalent GFP (MEG)
Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot.
The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted.
Standard curve
[Fluorescence intensity] = 0.0025*[GFP copies/cell]-14.416
R^2 = 0.9684
FP-0852-2 MEG
From the standard curve above, FP-0852-2 beads represent ~370,000 MEG (+/-40%)
