MEG

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(Molecules of Equivalent GFP (MEG))
(Molecules of Equivalent GFP (MEG))
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==Molecules of Equivalent GFP (MEG)==
==Molecules of Equivalent GFP (MEG)==
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Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot.
Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot.
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[[Image:FP-0852-2 MEG calibration.pdf]]
[[Image:FP-0852-2 MEG calibration.pdf]]
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The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted.
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===Standard curve===
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[Fluorescence intensity] = 0.0025*[GFP copies/cell]-14.416
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R^2 = 0.9684
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===FP-0852-2 MEG===
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From the standard curve above, FP-0852-2 beads represent ~370,000 MEG (+/-40%)

Revision as of 18:18, 29 November 2005

Molecules of Equivalent GFP (MEG)

Spherotech yellow fluorescent particles (FP-0852-2) were calibrated to GFP copies per cell as measured by quanitiative western blot.

Image:FP-0852-2 MEG calibration.pdf


The data series "Cells" includes samples from four independent chemostat cultures. Each contained MC4100 with a plasmid-based GFP generator: pSB4A3-I7109, pSB4A3-I7107, pSB3K3-I7109 or pSB3K3-I7107, as noted.

Standard curve

[Fluorescence intensity] = 0.0025*[GFP copies/cell]-14.416

R^2 = 0.9684

FP-0852-2 MEG

From the standard curve above, FP-0852-2 beads represent ~370,000 MEG (+/-40%)

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