M9 medium/supplemented: Difference between revisions
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==Specific | ==Specific Recipes== | ||
*[[Knight:M9 supplemented media|Knight | ===Knight & Endy Lab=== | ||
*[[Endy:M9 media/supplemented|Endy: | *[[M9 medium/minimal|M9 minimal medium]] supplemented with 0.2% Casamino acids and 1mM Thiamine Hydrochloride. | ||
**[[Knight:M9 supplemented media|Knight lab protocol]] | |||
**[[Endy:M9 media/supplemented|Endy lab protocol]] | |||
***[[Julius B. Lucks/M9 Supplemented Minimal Media|Lucks modified Endy recipe]] | |||
***[[User:Dileep_D._Monie/Recipes/Supplemented_M9|Monie modified Endy recipe]] | |||
[[Category:Material]] [[Category:Escherichia coli]] | |||
Latest revision as of 20:06, 8 April 2008
M9 medium also exists in various flavors:
M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts. [This and following instructions refer to the basic M9 medium recipe.]
M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.
M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.
Specific Recipes
Knight & Endy Lab
- M9 minimal medium supplemented with 0.2% Casamino acids and 1mM Thiamine Hydrochloride.
