M9 medium

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(Specific Protocols)
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*[[M9 media/supplemented|M9 supplmented media]]
*[[M9 media/supplemented|M9 supplmented media]]
&bull; [[M9 supplemented media|<font style="color:#000000">M9 supplemented media</font>]]  <BR>
&bull; [[M9 supplemented media|<font style="color:#000000">M9 supplemented media</font>]]  <BR>
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M9 medium also exists in various flavors:
 
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'''M9-glu''' contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts.
 
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'''M9-CA''' contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.
 
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'''M9-CAglu''' contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.
 
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''Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.''
 

Revision as of 10:19, 30 March 2006

Introduction

M9 media is a minimal media used for bacterial cultures. It has the advantage of being cheap and has a very low autofluorescence. M9 media can be supplemented to produce higher growth rates.


Specific Protocols

M9 supplemented media

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