M9 medium

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We used to prepare the '''plain M9 medium''' as follows:
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==Introduction==
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M9 media is a minimal media used for bacterial cultures.  It has the advantage of being cheap and has a very low autofluorescence.  M9 media can be supplemented to produce higher growth rates.
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{|
 
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| 900 ml sterile water
 
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| 100 ml M9 salts stock solution
 
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| 1 ml autoclaved 1 M MgSO<sub>4</sub>
 
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| 0.1 ml autoclaved 1 M CaCl<sub>2</sub>
 
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|}
 
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==Specific Protocols==
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*[[M9 media/minimal|M9 minimal media]]
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'''M9 stock solution''' (10x) is:
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*[[M9 media/supplemented|M9 supplmented media]]
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{|
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&bull; [[M9 supplemented media|<font style="color:#000000">M9 supplemented media</font>]]  <BR>
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| 60 g Na<sub>2</sub>HPO<sub>4</sub> x 7 H<sub>2</sub>O
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| 30 g KH<sub>2</sub>PO<sub>4</sub>
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| 5 g NaCl
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| 10 g NH<sub>4</sub>Cl
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| add water to 1000 ml and autoclave
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|}
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Revision as of 09:18, 30 March 2006

Introduction

M9 media is a minimal media used for bacterial cultures. It has the advantage of being cheap and has a very low autofluorescence. M9 media can be supplemented to produce higher growth rates.


Specific Protocols

M9 supplemented media


M9 medium also exists in various flavors:

M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts.

M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.

M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.

Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.

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