M9 medium

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We used to prepare the '''plain M9 medium''' as follows:
We used to prepare the '''plain M9 medium''' as follows:
-
<table>
+
 
-
<tr>900 ml sterile water</tr>
+
{|
-
<tr>100 ml M9 salts stock solution</tr>
+
| 900 ml sterile water
-
<tr>1 ml autoclaved 1 M MgSO4</tr>
+
|-
-
<tr>0.1 ml autoclaved 1 M CaCl2</tr>
+
| 100 ml M9 salts stock solution
-
</table>
+
|-
 +
| 1 ml autoclaved 1 M MgSO4
 +
|-
 +
| 0.1 ml autoclaved 1 M CaCl2
 +
|}
 +
 
'''M9 stock solution''' (10x) is:
'''M9 stock solution''' (10x) is:
-
<table>
+
{|
-
<tr>60 g Na2HPO4 x 7 H2O</tr>
+
| 60 g Na2HPO4 x 7 H2O
-
<tr>30 g KH2PO4</tr>
+
|-
-
<tr>5 g NaCl</tr>
+
| 30 g KH2PO4
-
<tr>10 g NH4Cl</tr>
+
|-
-
<tr>water to 1000 ml</tr></table>
+
| 5 g NaCl
-
autoclaved
+
|-
 +
| 10 g NH4Cl
 +
|-
 +
| add water to 1000 ml and autoclave
 +
|}
 +
 
M9 medium also exists in various flavors:
M9 medium also exists in various flavors:

Revision as of 04:41, 30 March 2006

We used to prepare the plain M9 medium as follows:

900 ml sterile water
100 ml M9 salts stock solution
1 ml autoclaved 1 M MgSO4
0.1 ml autoclaved 1 M CaCl2


M9 stock solution (10x) is:

60 g Na2HPO4 x 7 H2O
30 g KH2PO4
5 g NaCl
10 g NH4Cl
add water to 1000 ml and autoclave


M9 medium also exists in various flavors:

M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts.

M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.

M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.

Warning: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.

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