Lum: Difference between revisions

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(New page: ==Procedures== ===Quantification=== # '''Instument''' Qubit Fluoromete # '''Setting''' Broad Range #* Use Broad Range Standards in BR kit # Turn lights off, reagents are light sensitive #...)
 
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==Procedures==
==Research interests==
===Quantification===


# '''Instument''' Qubit Fluoromete
# [Microbial Community Analysis]: Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant “lupines chamissonis”
# '''Setting''' Broad Range
# [Tommy]
#* Use Broad Range Standards in BR kit
# [Jodie]
# Turn lights off, reagents are light sensitive
# [Elise]
# Prepare '''Working Solution'''
# [Amanda]
#* 199 µl '''Quant iT Buffer''' : 1 µl '''Quant iT Reagent'''
#*199:1 for every sample needed (ex.10 samples will be 2000 µl total)
# Prepare Samples
# *190 µl working solution + 10 µl standard 1 solution
# *190 µl working solution + 10 µl standard 2 solution
# *195 µl working solution + 5 µl of DNA
# Vortex for 2-3 minutes
# Incubate 15 minutes
# Follow On Screen Directions

Revision as of 22:02, 13 September 2010

Research interests

  1. [Microbial Community Analysis]: Bacterial and Fungal Diversity Associated with the Rhizosphere of the nitrogen fixing plant “lupines chamissonis”
  2. [Tommy]
  3. [Jodie]
  4. [Elise]
  5. [Amanda]
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