Luckau Protocols:PCR Buffers A-H: Difference between revisions
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(New page: {|{{table}} width="900" |- | width="600" style="background-color: #BCED91;" align="center"| <span style="font-size:32px;"> PCR Buffers A-H </span> |align="left" style="background-color: #...) |
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Revision as of 16:34, 25 September 2012
PCR Buffers A-H |
Purpose
PCR Buffer provides a stable chemical environment for polymerase during PCR. The buffer contains Tris (pH buffer, because DNA hydrolyzes in acidic conditions), MgCl2 (the divalent cation Mg++ is a cofactor for polymerase; the positively charged Mg++ stabilizes negatively charged DNA) and KCl (the positively charged K+ stabilizes negatively charged DNA).
Materials
Tris-Cl
- stuff
MgCl2
- stuff:
- and more stuff
KCl
- things
Protocol
Calculations
- math
Mix it all together
- stuff
Information?
- stuff