Luckau Protocols:FragmentAnalysisSubmission: Difference between revisions
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(New page: {|{{table}} width="900" |- | width="600" style="background-color: #BCED91;" align="center"| <span style="font-size:32px;"> Fragment Analysis Submission </span> |align="left" style="backgr...) |
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# Log-in to [http://services.arl.arizona.edu services.arl.arizona.edu] | # Log-in to [http://services.arl.arizona.edu services.arl.arizona.edu] | ||
# Click "Create Submission" (at bottom of page) | # Click "Create Submission" (at bottom of page) | ||
#: Click "Fragment Analysis / STR Typing" | #: - Click "Fragment Analysis / STR Typing" | ||
#: Click Account Number "111111" | #: - Click Account Number "111111" | ||
# In the "Grid Map" panel, click "Paste Sample Map" | # In the "Grid Map" panel, click "Paste Sample Map" | ||
#: copy sample grid from Excel spreadsheet | #: - copy sample grid from Excel spreadsheet | ||
#:: use only alpha numeric characters as well as "-" and "_" for special characters | #:: use only alpha numeric characters as well as "-" and "_" for special characters | ||
#:: sample names must be unique | #:: sample names must be unique | ||
# In the "Fragment Grid" panel: | # In the "Fragment Grid" panel: | ||
#: Dye Set: | #: - Dye Set: | ||
#:: G5 (5-dye) - | #:: G5 (5-dye) - UAGC will load ladder into the orange (ROX) color lane | ||
#:: D (4-dye) - | #:: D (4-dye) - UAGC will load ladder into the red (PET) color lane | ||
#: Dilution Ratio: | #: - Dilution Ratio: | ||
#:: 1/20 - this is standard; you shouldn't have to alter this | #:: 1/20 - this is standard; you shouldn't have to alter this | ||
# Click "Submit Grid" | # Click "Submit Grid" | ||
| Line 41: | Line 41: | ||
===Ship Order=== | ===Ship Order=== | ||
# write the four-letter submission ID onto your plate | # write the four-letter submission ID onto your plate | ||
# follow [ | #: this is how the lab technicians match the online order to the samples mailed | ||
# wrap plate in old FedEx bubble mailers to protect plate from light and the inherent bashing around that happens in the shipping process | |||
# follow [http://openwetware.org/wiki/Luckau_Protocols:ShipDryIce shipping] protocol | |||
Revision as of 14:54, 2 August 2012
| Fragment Analysis Submission |
Purpose
This protocol outlines how to submit samples for fragment analysis at the University of Arizona Genetics Core (samples are light-sensitive PCR product).
Protocol
Submit Order Online
- Log-in to services.arl.arizona.edu
- Click "Create Submission" (at bottom of page)
- - Click "Fragment Analysis / STR Typing"
- - Click Account Number "111111"
- In the "Grid Map" panel, click "Paste Sample Map"
- - copy sample grid from Excel spreadsheet
- use only alpha numeric characters as well as "-" and "_" for special characters
- sample names must be unique
- - copy sample grid from Excel spreadsheet
- In the "Fragment Grid" panel:
- - Dye Set:
- G5 (5-dye) - UAGC will load ladder into the orange (ROX) color lane
- D (4-dye) - UAGC will load ladder into the red (PET) color lane
- - Dilution Ratio:
- 1/20 - this is standard; you shouldn't have to alter this
- - Dye Set:
- Click "Submit Grid"
- Log the four-letter submission ID
- "Submission: frg-YY-MM-DD-XXXX"
Ship Order
- write the four-letter submission ID onto your plate
- this is how the lab technicians match the online order to the samples mailed
- wrap plate in old FedEx bubble mailers to protect plate from light and the inherent bashing around that happens in the shipping process
- follow shipping protocol
