Lucia I. Ramirez Week 10

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Biological Terms

  1. myc epitope tag:provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins (http://www.pierce-antibodies.com/Myc-Epitope-Tag-antibody-clone-MycA7-Monoclonal--MA121316.html)
  2. physiologic: of, or pertaining to physiology or normal functioning or state of an organism (http://www.biology-online.org/dictionary/Physiologic)
  3. immunoblot analysis: is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. It involves the solubilization and electrophoretic separation of proteins, glycoproteins, or lipopolysaccharides by gel electrophoresis, followed by quantitative transfer and irreversible binding to nitrocellulose, PVDF, or nylon (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583035/)
  4. immunoprecipitated: is the small-scale affinity purification of antigens using a specific antibody and is one of the most widely used methods for antigen purification and detection (https://www.lifetechnologies.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/immunoprecipitation-ip.html)
  5. stringency: reaction conditions, notably temperature, salt, and pH that dictate the annealing of single-stranded DNA/DNA, DNA/ rNA, and RNA/RNA hybrids. at high stringency, duplexes form only between strands with perfect one-to-one complementarity, lower stringency allows annealing between strands with some degree of mismatch between bases (http://www.biology-online.org/dictionary/Stringency)
  6. biosynthetic: The production of a complex chemical compound from simpler precursors in a living organism, usually involving enzymes (to catalyze the reaction) and energy source (such as ATP) (http://www.biology-online.org/dictionary/Biosynthesis)
  7. pheromone: chemical substances which, when secreted by an individual into the environment, cause specific reactions in other individuals, usually of the same species. The substances relate only to multicellular organisms (http://www.biology-online.org/dictionary/Pheromones)
  8. transient
  9. ultrasensitivity
  10. assaying

Outline

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Journals/Assignments

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Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

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