Loading dye: Difference between revisions
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Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels. | Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels. | ||
==Other dyes== | |||
*Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose) | |||
*Xylene cyanol: blue, runs at ~4kb | |||
Revision as of 15:23, 31 July 2006
Loading dye
- Ficoll 400 (in 68-558F)
- Deionized water
- Orange G dye
Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4°C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.
Other dyes
- Bromophenol blue: purple, generally runs at ~500bp (depending on percentage agarose)
- Xylene cyanol: blue, runs at ~4kb
