List of UNSes: Difference between revisions
No edit summary |
No edit summary |
||
Line 3: | Line 3: | ||
All current UNSes have been designed to minimize secondary structure under isothermal assembly conditions, to minimize cross-annealing to one another, and to minimize biological activity (no start codons, bacterial promoter-like sequences, or sequences with high BLAST scores to the E. coli genome). These UNSes were generated algorithmically [http://nar.oxfordjournals.org/content/42/1/681.long as described previously.] | All current UNSes have been designed to minimize secondary structure under isothermal assembly conditions, to minimize cross-annealing to one another, and to minimize biological activity (no start codons, bacterial promoter-like sequences, or sequences with high BLAST scores to the E. coli genome). These UNSes were generated algorithmically [http://nar.oxfordjournals.org/content/42/1/681.long as described previously.] | ||
U1: CATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTC | |||
U2: GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGC | |||
U3: GCACTGAAGGTCCTCAATCGCACTGGAAACATCAAGGTCG | |||
U4: CTGACCTCCTGCCAGCAATAGTAAGACAACACGCAAAGTC | |||
U5: GAGCCAACTCCCTTTACAACCTCACTCAAGTCCGTTAGAG | |||
U6: CTCGTTCGCTGCCACCTAAGAATACTCTACGGTCACATAC | |||
U7: CAAGACGCTGGCTCTGACATTTCCGCTACTGAACTACTCG | |||
U8: CCTCGTCTCAACCAAAGCAATCAACCCATCAACCACCTGG | |||
U9: GTTCCTTATCATCTGGCGAATCGGACCCACAAGAGCACTG | |||
UX: CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC | |||
Revision as of 11:30, 22 April 2014
List of Already-Designed UNSes
All current UNSes have been designed to minimize secondary structure under isothermal assembly conditions, to minimize cross-annealing to one another, and to minimize biological activity (no start codons, bacterial promoter-like sequences, or sequences with high BLAST scores to the E. coli genome). These UNSes were generated algorithmically as described previously.
U1: CATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTC U2: GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGC U3: GCACTGAAGGTCCTCAATCGCACTGGAAACATCAAGGTCG U4: CTGACCTCCTGCCAGCAATAGTAAGACAACACGCAAAGTC U5: GAGCCAACTCCCTTTACAACCTCACTCAAGTCCGTTAGAG U6: CTCGTTCGCTGCCACCTAAGAATACTCTACGGTCACATAC U7: CAAGACGCTGGCTCTGACATTTCCGCTACTGAACTACTCG U8: CCTCGTCTCAACCAAAGCAATCAACCCATCAACCACCTGG U9: GTTCCTTATCATCTGGCGAATCGGACCCACAAGAGCACTG UX: CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC