List of UNSes: Difference between revisions
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All current UNSes have been designed to minimize secondary structure under isothermal assembly conditions, to minimize cross-annealing to one another, and to minimize biological activity (no start codons, bacterial promoter-like sequences, or sequences with high BLAST scores to the E. coli genome). These UNSes were generated algorithmically [http://nar.oxfordjournals.org/content/42/1/681.long as described previously.] | All current UNSes have been designed to minimize secondary structure under isothermal assembly conditions, to minimize cross-annealing to one another, and to minimize biological activity (no start codons, bacterial promoter-like sequences, or sequences with high BLAST scores to the E. coli genome). These UNSes were generated algorithmically [http://nar.oxfordjournals.org/content/42/1/681.long as described previously.] | ||
U1: CATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTC | <br> | ||
U2: GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGC | '''U1:''' CATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTC | ||
U3: GCACTGAAGGTCCTCAATCGCACTGGAAACATCAAGGTCG | |||
U4: CTGACCTCCTGCCAGCAATAGTAAGACAACACGCAAAGTC | '''U2:''' GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGC | ||
U5: GAGCCAACTCCCTTTACAACCTCACTCAAGTCCGTTAGAG | |||
U6: CTCGTTCGCTGCCACCTAAGAATACTCTACGGTCACATAC | '''U3:''' GCACTGAAGGTCCTCAATCGCACTGGAAACATCAAGGTCG | ||
U7: CAAGACGCTGGCTCTGACATTTCCGCTACTGAACTACTCG | |||
U8: CCTCGTCTCAACCAAAGCAATCAACCCATCAACCACCTGG | '''U4:''' CTGACCTCCTGCCAGCAATAGTAAGACAACACGCAAAGTC | ||
U9: GTTCCTTATCATCTGGCGAATCGGACCCACAAGAGCACTG | |||
UX: CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC | '''U5:''' GAGCCAACTCCCTTTACAACCTCACTCAAGTCCGTTAGAG | ||
'''U6:''' CTCGTTCGCTGCCACCTAAGAATACTCTACGGTCACATAC | |||
'''U7:''' CAAGACGCTGGCTCTGACATTTCCGCTACTGAACTACTCG | |||
'''U8:''' CCTCGTCTCAACCAAAGCAATCAACCCATCAACCACCTGG | |||
'''U9:''' GTTCCTTATCATCTGGCGAATCGGACCCACAAGAGCACTG | |||
'''UX:''' CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC | |||
Latest revision as of 11:32, 22 April 2014
List of Already-Designed UNSes
All current UNSes have been designed to minimize secondary structure under isothermal assembly conditions, to minimize cross-annealing to one another, and to minimize biological activity (no start codons, bacterial promoter-like sequences, or sequences with high BLAST scores to the E. coli genome). These UNSes were generated algorithmically as described previously.
U1: CATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTC
U2: GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGC
U3: GCACTGAAGGTCCTCAATCGCACTGGAAACATCAAGGTCG
U4: CTGACCTCCTGCCAGCAATAGTAAGACAACACGCAAAGTC
U5: GAGCCAACTCCCTTTACAACCTCACTCAAGTCCGTTAGAG
U6: CTCGTTCGCTGCCACCTAAGAATACTCTACGGTCACATAC
U7: CAAGACGCTGGCTCTGACATTTCCGCTACTGAACTACTCG
U8: CCTCGTCTCAACCAAAGCAATCAACCCATCAACCACCTGG
U9: GTTCCTTATCATCTGGCGAATCGGACCCACAAGAGCACTG
UX: CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC
