List of UNSes: Difference between revisions

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All current UNSes have been designed to minimize secondary structure under isothermal assembly conditions, to minimize cross-annealing to one another, and to minimize biological activity (no start codons, bacterial promoter-like sequences, or sequences with high BLAST scores to the E. coli genome). These UNSes were generated algorithmically [http://nar.oxfordjournals.org/content/42/1/681.long as described previously.]
All current UNSes have been designed to minimize secondary structure under isothermal assembly conditions, to minimize cross-annealing to one another, and to minimize biological activity (no start codons, bacterial promoter-like sequences, or sequences with high BLAST scores to the E. coli genome). These UNSes were generated algorithmically [http://nar.oxfordjournals.org/content/42/1/681.long as described previously.]


{| class="wikitable" style="text-align:center; width:200px; height:200px; border: 1px solid darkgray"
<br>
|+ Ten UNSes
'''U1:''' CATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTC
|-
 
! ×
'''U2:''' GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGC
! 1
 
|-
'''U3:''' GCACTGAAGGTCCTCAATCGCACTGGAAACATCAAGGTCG
! 1
 
| 1
'''U4:''' CTGACCTCCTGCCAGCAATAGTAAGACAACACGCAAAGTC
|-
 
! 2
'''U5:''' GAGCCAACTCCCTTTACAACCTCACTCAAGTCCGTTAGAG
| 2
 
|-
'''U6:''' CTCGTTCGCTGCCACCTAAGAATACTCTACGGTCACATAC
! 3
 
| 3
'''U7:''' CAAGACGCTGGCTCTGACATTTCCGCTACTGAACTACTCG
|-
 
! 4
'''U8:''' CCTCGTCTCAACCAAAGCAATCAACCCATCAACCACCTGG
| 4
 
|-
'''U9:''' GTTCCTTATCATCTGGCGAATCGGACCCACAAGAGCACTG
! 5
 
| 5
'''UX:''' CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC
|}

Latest revision as of 11:32, 22 April 2014

List of Already-Designed UNSes

All current UNSes have been designed to minimize secondary structure under isothermal assembly conditions, to minimize cross-annealing to one another, and to minimize biological activity (no start codons, bacterial promoter-like sequences, or sequences with high BLAST scores to the E. coli genome). These UNSes were generated algorithmically as described previously.


U1: CATTACTCGCATCCATTCTCAGGCTGTCTCGTCTCGTCTC

U2: GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGC

U3: GCACTGAAGGTCCTCAATCGCACTGGAAACATCAAGGTCG

U4: CTGACCTCCTGCCAGCAATAGTAAGACAACACGCAAAGTC

U5: GAGCCAACTCCCTTTACAACCTCACTCAAGTCCGTTAGAG

U6: CTCGTTCGCTGCCACCTAAGAATACTCTACGGTCACATAC

U7: CAAGACGCTGGCTCTGACATTTCCGCTACTGAACTACTCG

U8: CCTCGTCTCAACCAAAGCAATCAACCCATCAACCACCTGG

U9: GTTCCTTATCATCTGGCGAATCGGACCCACAAGAGCACTG

UX: CCAGGATACATAGATTACCACAACTCCGAGCCCTTCCACC

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