Lissa1: August6-August14: Difference between revisions
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==August 7== | ==August 7== | ||
#Pour new SUMO gel | #Pour new SUMO gel -DONE | ||
#Pour new small gels | #Pour new small gels -DONE | ||
#Set up overnights for the following experiments: | #Set up overnights for the following experiments: | ||
##Nocodazole arrest | ##Nocodazole arrest -DONE | ||
##Induction experiment | ##Induction experiment -DONE | ||
##Make more unarrested 403 for phosphatase Western | ##Make more unarrested 403 for phosphatase Western -DONE | ||
#Make media for the nocodazole arrest. | #Make media for the nocodazole arrest. -DONE | ||
#Plan EVERYTHING | #Plan EVERYTHING -CHECK | ||
##Cloning | ##Cloning | ||
##Finalized experiments | ##Finalized experiments | ||
##What to do about luminol/ECF deal? | ##What to do about luminol/ECF deal? - OOPS, stil don't know | ||
#Integrate new-found numbers/data in to model | #Integrate new-found numbers/data in to model -DONE | ||
##Maybe update model to include Michaelis-Menton kinetics | ##Maybe update model to include Michaelis-Menton kinetics -NOT YET, talk to Ty | ||
==August 8== | ==August 8== |
Revision as of 08:14, 9 August 2006
August 6
August 7
- Pour new SUMO gel -DONE
- Pour new small gels -DONE
- Set up overnights for the following experiments:
- Nocodazole arrest -DONE
- Induction experiment -DONE
- Make more unarrested 403 for phosphatase Western -DONE
- Make media for the nocodazole arrest. -DONE
- Plan EVERYTHING -CHECK
- Cloning
- Finalized experiments
- What to do about luminol/ECF deal? - OOPS, stil don't know
- Integrate new-found numbers/data in to model -DONE
- Maybe update model to include Michaelis-Menton kinetics -NOT YET, talk to Ty
August 8
- Do nocodazole arrest! -CELLS WON'T GROW:(
- Do induction experiment! -DONE
- Set up overnights for the following experiments: -MOVED UNTIL TOMORROW
- Fus3/Active Fus3 gels
- Phosphatase control
- PCR up pGEV out of ACLY700 -DONE
- Run a gel of the PCR -DONE
- If there's time, run a second gel and extract and purify the pGEV (which might not be pGEV, based on the sequencing data) -DONE
August 9
- PCR amplify the pGEV band I purified yesterday
- Digest pGEV
- Setup overnight of pRS-405 in DH5alpha, in LB + Amp
- Prep phosphatase and Fus3/Active Fus3 samples - MOVED TO TOMORROW
- Load samples. Start SUMO. - MOVED TO TOMORROW
- Run Fus3/ Active Fus3 gel, and set up transfer. -MOVED TO TOMORROW
- Unfortunately, I've got a cold and need to rest:(
August 10
- Long incubation, wash, image Fus3 gel.
August 11
- Cut SUMO gel, set up transfer.
August 12
- Wash and image SUMO gel.