Lissa1: August6-August14: Difference between revisions
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==August 9== | ==August 9== | ||
#Prep phosphatase and Fus3/Active Fus3 samples | #PCR amplify the pGEV band I purified yesterday | ||
#Load samples. Start SUMO. | #Digest pGEV | ||
#Run Fus3/ Active Fus3 gel, and set up transfer. | #Setup overnight of pRS-405 in DH5alpha, in LB + Amp | ||
#Prep phosphatase and Fus3/Active Fus3 samples - MOVED TO TOMORROW | |||
#Load samples. Start SUMO. - MOVED TO TOMORROW | |||
#Run Fus3/ Active Fus3 gel, and set up transfer. -MOVED TO TOMORROW | |||
*Unfortunately, I've got a cold and need to rest:( | |||
==August 10== | ==August 10== |
Revision as of 08:14, 9 August 2006
August 6
August 7
- Pour new SUMO gel
- Pour new small gels
- Set up overnights for the following experiments:
- Nocodazole arrest
- Induction experiment
- Make more unarrested 403 for phosphatase Western
- Make media for the nocodazole arrest.
- Plan EVERYTHING
- Cloning
- Finalized experiments
- What to do about luminol/ECF deal?
- Integrate new-found numbers/data in to model
- Maybe update model to include Michaelis-Menton kinetics
August 8
- Do nocodazole arrest! -CELLS WON'T GROW:(
- Do induction experiment! -DONE
- Set up overnights for the following experiments: -MOVED UNTIL TOMORROW
- Fus3/Active Fus3 gels
- Phosphatase control
- PCR up pGEV out of ACLY700 -DONE
- Run a gel of the PCR -DONE
- If there's time, run a second gel and extract and purify the pGEV (which might not be pGEV, based on the sequencing data) -DONE
August 9
- PCR amplify the pGEV band I purified yesterday
- Digest pGEV
- Setup overnight of pRS-405 in DH5alpha, in LB + Amp
- Prep phosphatase and Fus3/Active Fus3 samples - MOVED TO TOMORROW
- Load samples. Start SUMO. - MOVED TO TOMORROW
- Run Fus3/ Active Fus3 gel, and set up transfer. -MOVED TO TOMORROW
- Unfortunately, I've got a cold and need to rest:(
August 10
- Long incubation, wash, image Fus3 gel.
August 11
- Cut SUMO gel, set up transfer.
August 12
- Wash and image SUMO gel.