Lissa1: August6-August14

August 7

1. Pour new SUMO gel
2. Pour new small gels
3. Set up overnights for the following experiments:
   1. Nocodazole arrest
   2. Induction experiment
   3. Make more unarrested 403 for phosphatase Western
4. Make media for the nocodazole arrest.
5. Plan EVERYTHING
   1. Cloning
   2. Finalized experiments
   3. What to do about luminol/ECF deal?
6. Integrate new-found numbers/data in to model
   1. Maybe update model to include Michaelis-Menton kinetics

August 8

1. Do nocodazole arrest!
2. Do induction experiment!
3. Set up overnights for the following experiments:
   1. Fus3/Active Fus3 gels
   2. Phosphatase control
4. PCR up pGEV out of ACLY700
5. Run a gel of the PCR
6. If there's time, run a second gel and extract and purify the pGEV (which might not be pGEV, based on the sequencing data)

August 9

1. Prep phosphatase and Fus3/Active Fus3 samples
2. Load samples. Start SUMO.
3. Run Fus3/ Active Fus3 gel, and set up transfer.
4. Set up start of cloning stuff. (PCR pGEV, digest, etc)

August 10

1. Long incubation, wash, image Fus3 gel.

August 11

1. Cut SUMO gel, set up transfer.

August 12

1. Wash and image SUMO gel.