Lissa1: August6-August14: Difference between revisions
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##Nocodazole arrest | ##Nocodazole arrest | ||
##Induction experiment | ##Induction experiment | ||
##Make more unarrested 403 for phosphatase Western | |||
#Make media for the nocodazole arrest. | #Make media for the nocodazole arrest. | ||
#Plan EVERYTHING | |||
##Cloning | |||
##Finalized experiments | |||
##What to do about luminol/ECF deal? | |||
#Integrate new-found numbers/data in to model | |||
##Maybe update model to include Michaelis-Menton kinetics | |||
==August 8== | ==August 8== | ||
#Do nocodazole arrest! | #Do nocodazole arrest! |
Revision as of 11:14, 7 August 2006
August 6
August 7
- Pour new SUMO gel
- Pour new small gels
- Set up overnights for the following experiments:
- Nocodazole arrest
- Induction experiment
- Make more unarrested 403 for phosphatase Western
- Make media for the nocodazole arrest.
- Plan EVERYTHING
- Cloning
- Finalized experiments
- What to do about luminol/ECF deal?
- Integrate new-found numbers/data in to model
- Maybe update model to include Michaelis-Menton kinetics
August 8
- Do nocodazole arrest!
- Do induction experiment!
- Set up overnights for the following experiments:
- Fus3/Active Fus3 gels
- Phosphatase control
August 9
- Prep phosphatase and Fus3/Active Fus3 samples
- Load samples. Start SUMO.
- Run Fus3/ Active Fus3 gel, and set up transfer.
- Set up start of cloning stuff. (PCR pGEV, digest, etc)
August 10
- Long incubation, wash, image Fus3 gel.
August 11
- Cut SUMO gel, set up transfer.
August 12
- Wash and image SUMO gel.