Lissa1: August6-August14: Difference between revisions
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#Load samples. Start SUMO. | #Load samples. Start SUMO. | ||
#Run Fus3/ Active Fus3 gel, and set up transfer. | #Run Fus3/ Active Fus3 gel, and set up transfer. | ||
#Set up start of cloning stuff. (PCR pGEV, digest, etc) | |||
==August 10== | ==August 10== | ||
#Long incubation, wash, image Fus3 gel. | #Long incubation, wash, image Fus3 gel. | ||
Revision as of 09:56, 7 August 2006
August 6
August 7
- Pour new SUMO gel
- Pour new small gels
- Set up overnights for the following experiments:
- Nocodazole arrest
- Induction experiment
- Make media for the nocodazole arrest.
August 8
- Do nocodazole arrest!
- Do induction experiment!
- Set up overnights for the following experiments:
- Fus3/Active Fus3 gels
- Phosphatase control
August 9
- Prep phosphatase and Fus3/Active Fus3 samples
- Load samples. Start SUMO.
- Run Fus3/ Active Fus3 gel, and set up transfer.
- Set up start of cloning stuff. (PCR pGEV, digest, etc)
August 10
- Long incubation, wash, image Fus3 gel.
August 11
- Cut SUMO gel, set up transfer.
August 12
- Wash and image SUMO gel.
