Lissa1:Pouring Plates

1. Get 200 mL of 4% agar.
2. Microwave agar.
3. From kitchen, get 2x SD 200 mL (synthetic dropout – nitrogen/vitamins/minerals).
4. Add liquid ingredients in SD flask.
5. Add 40 mL 10x CSM (minus stuff) – you are diluting it to 1x. These are the amino acids.
6. Add dextrose. It’s at 40%, you need to add 20 mL to make a 2% solution in 400 mL.
7. Pour stuff from SD flask in to agar jar, then pour plates.