Lidstrom: Agarose Gel Electrophoresis: Difference between revisions
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== Starting with an empty box == | == Starting with an empty box == | ||
* Fill with TBE until it covers the gel holding tray. | * Fill with TBE until it covers the gel holding tray. | ||
* Add 40 | * Add 40 uL of ethidium bromide if your gels don't already have it within | ||
** If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good. | ** If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good. | ||
* Load sample | * Load sample | ||
** 1.7 uL of a PCR product is usually plenty | |||
* Load ladder | * Load ladder | ||
** If using MassRuler, 4 uL is good for skinny lanes. | ** If using MassRuler, 4 uL is good for skinny lanes. | ||
* Run @ | * Run @ 110 V for 20 min and check the gel. | ||
** 40 min is pretty safe for 0.7 - 1% agarose gels | |||
Revision as of 20:51, 20 February 2013
Return to Protocols
- handy link: gel electrophoresis
- click the gel electrophoresis button on the left
Starting with an empty box
- Fill with TBE until it covers the gel holding tray.
- Add 40 uL of ethidium bromide if your gels don't already have it within
- If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.
- Load sample
- 1.7 uL of a PCR product is usually plenty
- Load ladder
- If using MassRuler, 4 uL is good for skinny lanes.
- Run @ 110 V for 20 min and check the gel.
- 40 min is pretty safe for 0.7 - 1% agarose gels
