Lidstrom: Agarose Gel Electrophoresis

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** click the gel electrophoresis button on the left
** click the gel electrophoresis button on the left
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* add ___ uL of ethidium bromide per mL of ethidium-bromide free TBE.
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== Starting with an empty box ==
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* Fill with TBE until it covers the gel holding tray.
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* Add 40 uL of ethidium bromide if your gels don't already have it within
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** If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.  
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* Load sample
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** 1.7 uL of a PCR product is usually plenty
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* Load ladder
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** If using MassRuler, 4 uL is good for skinny lanes.
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* Run @ 110 V for 20 min and check the gel.
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** 40 min is pretty safe for 0.7 - 1% agarose gels

Revision as of 23:51, 20 February 2013

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Starting with an empty box

  • Fill with TBE until it covers the gel holding tray.
  • Add 40 uL of ethidium bromide if your gels don't already have it within
    • If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.
  • Load sample
    • 1.7 uL of a PCR product is usually plenty
  • Load ladder
    • If using MassRuler, 4 uL is good for skinny lanes.
  • Run @ 110 V for 20 min and check the gel.
    • 40 min is pretty safe for 0.7 - 1% agarose gels
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