Lidstrom: Agarose Gel Electrophoresis: Difference between revisions
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** click the gel electrophoresis button on the left | ** click the gel electrophoresis button on the left | ||
* | == Starting with an empty box == | ||
* Fill with TBE until it covers the gel holding tray. | |||
* Add 40? uL of ethidium bromide if your gels don't already have it within | |||
** If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good. | |||
* Load sample | |||
* Load ladder | |||
** If using MassRuler, 4 uL is good for skinny lanes. | |||
* Run @ 100 V for 20 min and check the gel. | |||
Revision as of 19:19, 20 February 2013
Return to Protocols
- handy link: gel electrophoresis
- click the gel electrophoresis button on the left
Starting with an empty box
- Fill with TBE until it covers the gel holding tray.
- Add 40? uL of ethidium bromide if your gels don't already have it within
- If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.
- Load sample
- Load ladder
- If using MassRuler, 4 uL is good for skinny lanes.
- Run @ 100 V for 20 min and check the gel.
