Lidstrom:UV Mutagenesis

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Resources

• UV Radiation and Spontaneous Mutagenesis: summary by Kendric C. Smith, Emeritus Professor, Radiation Oncology (Radiation Biology), Stanford University School of Medicine
  • Discusses repair mechanisms a lot.
  • "G:C -> A:T transitions predominate after UV irradiation. In E. coli the (6-4)-photoproduct may be more important for mutagenesis, while the pyrimidine dimer may be more important in mammalian cells. In human cells, mutations occur at the C of a TC, CT, or CC pyrimidine dimer, but not at TT dimers, and also occur at the C of TC and CC (6-4)-adducts (reviewed by Brash, 1988)."
  • "Therefore, as with survival, no one UV-radiation-induced lesion can be assigned as the one and only mutagenic lesion. Under certain experimental conditions, and in certain base sequences within a gene, pyrimidine dimers can be the most important mutagenic lesions, however, under other experimental conditions, and at other base sequences (e.g., CC sites), the (6-4)-adduct can be the most important. Furthermore, purine photoproducts have been identified, and have been implicated in mutagenesis (reviewed in Brash, 1988). Therefore, statements about the relative importance of different photoproducts in lethality and mutagenesis must be assessed on an experiment by experiment basis. "